TRAP-SCAR252 (target region amplification polymorphism-sequence characterized amplified region 252) marker for identifying E group chromosomes of Agropyron elongatum and application thereof

Abstract

The invention discloses a TRAP-SCAR252 marker for identifying E group chromosomes of Agropyron elongatum and an application thereof. The nucleotide sequence of the marker is represented by SEQ ID NO.3 or SEQ ID NO.6. A specific TRAP fragment which is obtained by screening through a TRAP-SCAR method is converted into a stable SCAR marker, and the stable SCAR marker is amplified and detected. The marker of the E group chromosomes of Agropyron elongatum provided in the invention lays a good foundation for rapidly identifying 5E or 6E chromatins or chromosomes of Agropyron elongatum in the genetic background of wheat and simultaneously provides a very convenient and fast approach for the molecular marker assisted breeding through the SCAR tag detection.

Description

Technical field [0001] The present invention belongs to the technical field of identification of Elytrigia Elytrigia group E chromosomes, and relates to a TRAP-SCAR for identifying Elytrigia Elytrigia group E chromosomes 252 Marking and its application. Background technique [0002] Wheat is one of the most important food crops in the world. It is the second largest crop in China after rice, and wheat production is of very important strategic significance to the development of the national economy. Due to the simplification of wheat cultivars, its genetic basis is becoming increasingly narrow and the source of resistance is scarce, which greatly limits the further improvement of its yield and quality. There are a large number of genetic variations in the relative species of wheat, which are beneficial gene resources that can be used for wheat improvement. The introduction of these beneficial genes into wheat has always been an enduring research topic for geneticists and breeders...

AI Technical Summary

Problems solved by technology

[0005] Hybridization and introgression are an effective way to introduce the excellent traits and genes of exogenous chromosomes into wheat. The detection of chromatin or chromosomes has become a crucial issue. Although valuable genes have been mapped on the chromosomes of E. elongatum through agronomically important characteristics and biochemical analysis, the detection methods of these experiments are not stable, and Not suitable for testing important agronomic traits

Some genetic methods, such as chromosome banding and in situ hybridization, have been used to detect exogenous genetic material in the genetic background of wheat, but these techniques are time-consuming, laborious, and have high technical requirements. These experimental methods still have certain limitations for large-scale rapid screening of offspring.

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