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Promoter of specificity of heart leaves of Zea mays ssp. Mays L. and application of specific promoter in transgenic breeding

A promoter, specific technology, applied in the field of plant genetic engineering, can solve problems such as changing plant metabolism

Inactive Publication Date: 2012-03-14
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the safety of transgenic plants has always been the focus of attention, in which the expression of foreign genes may change the metabolism of the plant itself, thus bringing potential risks that are currently unknown

Method used

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  • Promoter of specificity of heart leaves of Zea mays ssp. Mays L. and application of specific promoter in transgenic breeding
  • Promoter of specificity of heart leaves of Zea mays ssp. Mays L. and application of specific promoter in transgenic breeding
  • Promoter of specificity of heart leaves of Zea mays ssp. Mays L. and application of specific promoter in transgenic breeding

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Embodiment Construction

[0017] The present invention will be further described in detail through the following examples in conjunction with the accompanying drawings, but the scope of the present invention will not be limited in any way.

[0018] This embodiment verified the specific high expression of the ZmPsbS1 gene in young maize heart leaves by real-time fluorescent quantitative PCR (FQ-PCR), thereby cloning its promoter, which is 1475bp in length, and its sequence is shown in SEQ ID No in the sequence listing :1.

[0019] 1. Materials

[0020] Maize (Zea mays ssp. Mays L.) was grown in a greenhouse at 26°C for 4 weeks, with 16 hours of light and 8 hours of darkness per day.

[0021] 2. Total RNA extraction and cDNA synthesis

[0022] 1. Extraction of total RNA from each tissue of maize

[0023] Take the roots, stems and heart leaves of 4-week-old corn, grind 2g of fresh and clean corn tissue into powder in liquid nitrogen, transfer to 1ml Trizol solution (Invitrogen Company), mix well, add 2...

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Abstract

The invention discloses a promoter of the specificity of heart leaves of Zea mays ssp. Mays L. and an application of the specific promoter in the transgenic breeding. The promoter which is a promoter of a ZmPsbS1 gene of Zea mays ssp. Mays L. has a nucleotide sequence represented by SEQ ID No:1 or a nucleotide sequence which can be hybridized with the nucleotide sequence represented by SEQ ID NO:1 under strict conditions and has same functions with the nucleotide sequence represented by SEQ ID NO:1. The promoter of the present invention, which has the specificity of heart tissues, can be applied to the field of transgenic plant breeding. By constructing a plant expression vector containing the promoter, the promoter is converted to plants (such as Zea mays ssp. Mays L.) to drive downstream genes to specifically express in the heart leaves of the plants, so the breeding purpose is reached.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering and relates to the cloning of a plant promoter and its application, in particular to the cloning of a corn heart-leaf specific promoter and its function analysis. Background technique [0002] Corn is not only the main crop for food and feed, but also an important industrial raw material. With the popularization and acceleration of industrialization, the contradictions between the decrease of arable land, the deterioration of the environment and the shortage of food have become increasingly acute, while conventional breeding techniques cannot meet human needs for high-yield, high-quality, disease-resistant and stress-resistant new corn varieties. For more than ten years, a batch of new varieties of corn that are insect-resistant, disease-resistant, herbicide-resistant, salt-resistant, drought-resistant, and high-quality have been bred using transgenic technology. [0003] Corn b...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 洪龙魏桐徐唯涛高远顾红雅瞿礼嘉
Owner PEKING UNIV
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