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SNPs (Single nucleotide polymorphisms) sites of SPAG6 (Sperm associated antigen 6) gene for screening chicken with excellent sperm quality, heplotype composition thereof and application of heplotype composition

A haplotype combination and genotype technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as sperm head breakage, sperm structure damage, couple damage, etc.

Active Publication Date: 2012-03-14
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In mice lacking Spag6 protein, the instability of centriole microtubules directly leads to the destruction of sperm structure, the sperm head is easily broken, and the peripheral dense fibers and peripheral microtubule couplets are also destroyed

Method used

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  • SNPs (Single nucleotide polymorphisms) sites of SPAG6 (Sperm associated antigen 6) gene for screening chicken with excellent sperm quality, heplotype composition thereof and application of heplotype composition
  • SNPs (Single nucleotide polymorphisms) sites of SPAG6 (Sperm associated antigen 6) gene for screening chicken with excellent sperm quality, heplotype composition thereof and application of heplotype composition
  • SNPs (Single nucleotide polymorphisms) sites of SPAG6 (Sperm associated antigen 6) gene for screening chicken with excellent sperm quality, heplotype composition thereof and application of heplotype composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Identification of SPAG6_SNP1-5 markers closely related to chicken semen quality and primers for detecting genotypes at SPAG6_SNP1-5 sites and compositions containing the primers

[0017] 1. Test data collection

[0018] 390 Beijing oil chicken roosters raised in Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences were selected. All experimental chickens were from the same batch hatched and reared under the same conditions. When the roosters were raised to 43 weeks old, an experimenter collected the semen by abdominal massage, collected it in a semen collection cup, and immediately measured the semen volume, sperm motility, sperm viability and sperm deformity rate.

[0019] 2. Extract the genomic DNA of individuals in the above groups

[0020] Genomic DNA of chicken peripheral blood cells was extracted by high-salt method.

[0021] (1) Digestion Add 30μl chicken anticoagulant blood to a 2ml centrifuge tube...

Embodiment 2

[0033] Example 2: Identification of haplotype combinations of SPAG6_SNP1-5 loci with good semen quality

[0034] 1. Haplotype construction

[0035] Input the genotype data of SPAG6_SNP1-5 loci of all individuals obtained through the above PCR-direct sequencing method into the PHASE program, and the haplotype of each individual can be calculated, and the degree of pairwise linkage disequilibrium between the loci can be calculated at the same time , represented by the normalized linkage disequilibrium coefficient D'.

[0036] The results of pairwise linkage disequilibrium analysis of SPAG6 gene 5 loci at SPAG6_SNP1-5 locus are shown in Table 2. The analysis results show that the linkage disequilibrium coefficient D' between SPAG6_SNP1 and SPAG6_SNP2, SPAG6_SNP4 and SPAG6_SNP5 base sites is 1, which is a complete linkage disequilibrium; the linkage disequilibrium between SPAG6_SNP1 (or SPAG6_SNP2) and SPAG6_SNP3 base sites is in a state of linkage instability. The linkage diseq...

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Abstract

The invention comprises 5 new SNP (Single nucleotide polymorphism)-labeled SPAG6 (Sperm associated antigen 6)_SNP1-5 closely associated with the sperm quality of chicken on the SPAG6 gene and a heplotype composition of SPAG6_SNP1-5 sites with excellent sperm quality. The invention also comprises a primer for detecting the genotypes of the SPAG6_SNP1-5 sites and the heplotype composition thereof, a composition comprising the primer and a method for screening the chicken with excellent sperm quality by utilizing the primer or the primer composition and heplotype composition. The SPAG6_SNP1-5 sites are the 20352nd-site, 20426th-site, 20496th-site, 20568th-site and 20586th-site base sites of chicken SPAG6 gene; and the heplotype composition is GCTCA / GCCCA, and the heplotype composition individuals are optimal in the aspects of sperm amount, sperm deformity rate and sperm motility rate.

Description

technical field [0001] The invention belongs to the technical field of molecular marker assisted breeding of agricultural breeding animals. Specifically, it involves five new SNP markers SPAG6_SNP1-5 on the SPAG6 gene that are closely related to chicken semen quality and a haplotype combination of SPAG6_SNP1-5 loci with excellent semen quality. The present invention also includes primers for detecting the SPAG6_SNP1-5 site genotype and its haplotype combination, a composition comprising the primer, and a method for screening chickens with excellent semen quality by using the above primer or primer composition and haplotype combination method. Background technique [0002] Semen quality is a comprehensive trait, and its quality not only reflects the reproductive status of male animals, but also relates to the fertilization ability of sperm. In livestock and poultry production, semen volume, sperm motility, sperm motility rate and sperm deformity rate are routine indicators ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈继兰胡娟文杰赵桂苹郑麦青刘冉冉
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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