Rapid propagation technique of Dendrobium candidum axillary buds

Abstract

The invention discloses a rapid propagation technique of Dendrobium candidum axillary buds. According to the technique, Dendrobium candidum axillary buds are used as explants directly to induce fasciculated buds; influences of combinations of different types of hormones with different concentration and different natural extracts on survival and growth of axillary buds, induction and multiplication of fasciculated buds and seedlings are compared, the quantity and quality of seedlings growing from fasciculated buds are researched and explored, the optimal culture medium formula in each stage is screened, and therefore, a fasciculated-bud-way high-efficiency rapid propagation system is established, so that plants obtained through rapid propagation are consistent with parent plants genetically so as to provide basis for restoration of wild population of Dendrobium candidum, and furthermore, wild resources are protected. Compared with vegetative propagation, the rapid propagation technique provided by the invention greatly improves propagation factor. A high-efficiency rapid propagation system of Dendrobium candidum which is an endangered plant provides a technical probability for the large-scale production and is beneficial to the restoration of wild population.

Description

technical field [0001] The invention relates to a rapid propagation technology of plants, in particular to a rapid propagation technology of dendrobium officinale axillary buds, and belongs to the field of biotechnology. Background technique [0002] Dendrobium officinale Kimura et Migo or (Dendrobium candidum Wall ex Lind.), also known as black knot grass, belongs to Orchidaceae. Dendrobium is a perennial epiphytic herb, often epiphytic on tree trunks or rocks in dense forests, and often associated with bryophytes. Its fresh stem or dried stem is used as medicine, and it is the most commonly used raw material of traditional medicinal materials and Chinese patent medicines in my country. "Chinese Pharmacopoeia" stipulates that Dendrobium candidum can be processed into earrings, that is, Tiepi Fengdou, after drying and softening. Its taste is sweet and mild, and its nature is slightly cold. It is used for yin deficiency, dry mouth, polydipsia, retching due to lack of food, ...

AI Technical Summary

Problems solved by technology

The problem is that the offspring propagated by seeds may mutate; the stem tip will lose the mother plant as an explant, and the stem section will be incompletely sterilized due to its own serious bacterial infection

At present, there is no report on the direct cultivation of axillary buds of Dendrobium officinale to induce seedling formation