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Diluent for preserving rabbit sperms at normal temperature and preparation method thereof

A technology of preservation at room temperature and diluent, which is applied in the field of animal reproduction, can solve the problems that affect the popularization and application of artificial insemination technology of rabbits, cannot be popularized and applied in rural areas and pastoral areas, and the price of imported products is expensive, so as to achieve simple production, convenient use and storage , cost reduction effect

Inactive Publication Date: 2012-01-18
青岛康大食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The price of imported products is expensive, which is unaffordable for general rabbit raising units, and cannot be popularized and applied in China's vast rural and pastoral areas, which will seriously affect the promotion and application of rabbit artificial insemination technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: A diluent for preservation of rabbit semen at room temperature, consisting of 30g tris 30g, 15g citric acid, 6g glucose, 0.5g potassium chloride, 0.2g glutathione, and 0.05g kanamycin, It is prepared by adding double distilled water to 1L.

[0013] The preparation steps are as follows: (1) Weigh 30g tris, 150g citric acid, 6g glucose, 0.5g potassium chloride, 0.2g glutathione, 0.05g kanamycin, and add double distilled water. Dilute the volume to 1L and shake it up; (2) Dissolve the solution obtained in step (1) in a water bath at 35 degrees Celsius for 30 minutes to obtain the diluent with a pH of 6.8.

[0014] The prepared diluent is slowly added to the male rabbit semen, diluted to 5-15 times according to the quality of the semen, and the diluted semen is stored at 15-18 degrees Celsius for 0-48 hours.

[0015] When in use, 0.4-0.5 ml of diluted semen within the validity period is transferred into the uterus of a female rabbit to complete artificial insemination....

Embodiment 2

[0016] Example 2: A diluent for preservation of rabbit semen at room temperature, composed of 32g tris 32g, citric acid 16g, 8g glucose, 0.5g potassium chloride, 0.3g glutathione, and 0.06g kanamycin, It is prepared by adding double distilled water to 1L.

[0017] The preparation steps are as follows: (1) Weigh 32g of tris(hydroxymethyl)aminomethane, 16g of citric acid, 8g of glucose, 0.5g of potassium chloride, 0.3g of glutathione, 0.06g of kanamycin, and add double distilled water. Dilute the volume to 1L and shake it up; (2) Dissolve the solution obtained in step (1) in a water bath at 35 degrees Celsius for 30 minutes to obtain the diluted solution with a pH of 6.8.

[0018] The method of preservation and use of semen is the same as in the first embodiment.

Embodiment 3

[0019] Example 3: A diluent of rabbit semen preserved at room temperature, consisting of 34g tris 34g, citric acid 17g, glucose 10g, potassium chloride 0.6g, glutathione 0.3g, kanamycin 0.07g, It is prepared by adding double distilled water to 1L.

[0020] The preparation steps are as follows: (1) Weigh 34g tris, 17g citric acid, 10g glucose, 0.6g potassium chloride, 0.3g glutathione, 0.07g kanamycin, and add double distilled water. Dilute the volume to 1L and shake it up; (2) Dissolve the solution obtained in step (1) in a water bath at 35 degrees Celsius for 30 minutes to obtain the diluent with a pH of 6.8.

[0021] The method of preservation and use of semen is the same as in the first embodiment.

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PUM

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Abstract

The invention relates to a diluent for preserving rabbit sperms at normal temperature and a preparation method thereof and belongs to the technical field of animal reproduction. The diluent is prepared by adding water into 30-40g of tris(hydroxymethyl)aminomethane, 15-20g of citric acid, 6-15g of dextrose, 0.5-1g of potassium chloride, 0.2-0.5g of glutathione and 0.05-0.1g of kanamycin until the total volume reaches 1L; and the pH value of the diluent is 6.8-7.0. By using the diluent, the rabbit sperms can be preserved for a short time, and the sperm viability can be 0.7 and the acrosome integrity rate can be 87% after the rabbit sperms are preserved by using the diluent for 48 hours at normal temperature, thus the sperm viability and the acrosome integrity rate exceed the requirements of artificial insemination of rabbits, the conception rate is up to 89.4%, and the conception rate reaches or is superior to those obtained by using the similar import diluents. Meanwhile, the diluent provided by the invention is used and stored more conveniently, and the diluent can be instant availably at the time of artificial insemination, thus being convenient and quick. The diluent is prepared simply and can be used for lowering the cost of the artificial insemination of the rabbits.

Description

Technical field [0001] The invention relates to the technical field of animal reproduction, in particular to a diluent for preservation of rabbit semen at room temperature, which is used for artificial fertilization of rabbits. Background technique [0002] my country is the world's largest country for raising rabbits, but the efficiency of rabbit breeding in our country is only about 50% of that of developed countries in Europe. The low breeding efficiency restricts the development of the rabbit industry in China. The insufficient application and promotion of artificial insemination technology is an important factor leading to the low efficiency of the rabbit industry in my country. The preservation of semen is a bottleneck that affects the application and promotion of artificial insemination technology in rabbits. The artificial insemination technology of rabbits has the advantages of saving breeding costs, improving labor efficiency, reducing labor intensity, increasing concep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 李士栋焦德蒙崔钦阎英凯李明勇
Owner 青岛康大食品有限公司
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