Making method for producing biologic protein feeds by using corncobs
A technology of biological protein feed and production method, which is applied in the field of production of biological protein feed by using corncobs, can solve the problems such as the production method of biological protein feed that has not been seen, and achieve the effect of reducing rural environmental pollution and saving feed grain
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Embodiment 1
[0024] 1. Preparation of seed solution
[0025] (1) Aspergillus niger, preserved in the Biological Center of Yancheng Institute of Technology.
[0026] The slant medium is (Charles medium): NaNO 3 2g, K 2 HPO 4 1g, KCl 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 0.01g, 30g sucrose, 20g agar, distilled water to 1000mL, natural pH, sterilized at 121°C for 20 minutes. Inoculate Aspergillus niger and culture at 30°C for 72 hours to obtain slant strains.
[0027] The screening medium is: carboxymethyl cellulose sodium 20g, Na 2 HPO 4 2.5g, KH 2 PO 4 1.6g, 2.6g peptone, 0.8g yeast extract, 1000ml distilled water, 20g agar, neutral pH, sterilized at 121°C for 20 minutes. Inoculate the slant strain of Aspergillus niger, culture it at 30°C for 72 hours, and treat it with 1g / l Congo red, pick the strain with the largest bacterial circle, and obtain the screened Aspergillus niger.
[0028] Seed medium: NaNO 3 2g,K 2 HPO 4 1g, KCl 0.5g, MgSO 4 0.5g, FeSO 40.01g, 30g sucrose...
Embodiment 2
[0042] 1. Preparation of seed solution
[0043] (1) Aspergillus niger, preserved in the Biological Center of Yancheng Institute of Technology.
[0044] The slant medium is (Charles medium): NaNO 3 2g, K 2 HPO 4 1g, KCl 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 0.01g, 30g sucrose, 20g agar, distilled water to 1000mL, natural pH, sterilized at 121°C for 20 minutes. Inoculate Aspergillus niger and culture at 30°C for 72 hours to obtain slant strains.
[0045] The screening medium is: carboxymethyl cellulose sodium 20g, Na 2 HPO 4 2.5g, KH 2 PO 4 1.6g, 2.6g peptone, 0.8g yeast extract, 1000ml distilled water, 20g agar, neutral pH, sterilized at 121°C for 20 minutes. Inoculate the slant strain of Aspergillus niger, culture it at 30°C for 72 hours, and treat it with 1g / l Congo red, pick the strain with the largest bacterial circle, and obtain the screened Aspergillus niger.
[0046] Seed medium: NaNO 3 2g, K 2 HPO 4 1g, KCl 0.5g, MgSO 4 0.5g, FeSO 4 0.01g, 30g sucr...
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