Method and pair of special primers for identifying purple properties of Chinese cabbages

Abstract

The invention discloses a method and a pair of special primers for identifying purple properties of Chinese cabbages. One of the primers is shown in SEQ ID NO:1, and the other one of the primers is shown in SEQ ID NO:2. In the invention, by using the F2 colony for controlling the genes of the purple properties to be positioned on the A3 chromosome for the first time, molecular markers which are closely linked with the purple properties are found, and the molecular markers can be used for identifying the purple properties of Chinese cabbages, thereby laying the foundations for performing molecular marker-assisted breeding and cloning genes. The identification method disclosed by the invention has accurate and reliable identification results and can be used for identifying in the early stage of growth of Chinese cabbages without waiting for the maturation of plants, thereby saving the time, lowering the labor cost, increasing the breeding speed and having important significance.

Description

technical field [0001] The invention relates to a method for identifying purple traits of cabbage and a pair of special primers. Background technique [0002] Anthocyanins are water-soluble pigments that exist in plants. They consist of a basic structural core and different substituents. Due to the different types and positions of substituents, anthocyanins of different colors are formed, including pink, red, and purple. and blue, about 88% of the flower color of angiosperms is determined by anthocyanins, which are mainly accumulated in plant vacuoles and have a variety of physiological functions, such as attracting insects for pollination, resisting low temperature and ultraviolet damage, and Prevention and treatment of plant diseases, etc.; anthocyanin is the most powerful free radical scavenger found so far, with anti-oxidative aging, anti-mutation, anti-cancer and anti-arteriosclerosis functions; in addition, anthocyanin is a safe, non-toxic Toxic natural food coloring....

AI Technical Summary

Problems solved by technology

Although Zhang Mingke found a marker linked to the purple trait, its genetic distance is still relatively far away, far from meeting the requirements of molecular marker-assisted breeding

[0008] At present, among the applied labels, the amount of DNA required for RFLP labeling is large, the steps are many, and the cycle is long. Radioactive isotopes are used in the preparation of probes and detection, and the cost is high; RAPD labeling uses relatively short primers. The reaction conditions are extremely sensitive, a slight change will affect the reproduction of the amplified product, the repeatability is poor, and the stability is not good

In addition, RAPD is a dominant marker, which cannot distinguish homozygous genotypes from heterozygous genotypes, and cannot be directly used for genotype analysis; AFLP markers are expensive, and the method requires multi-step operations, which makes statistical analysis difficult and affects the purity of DNA. High quality requirements for endonucleases and endonucleases; SNPs provide less information and are more expensive

Images