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Co-immobilized TNF-a/IFN-g/DOX/FA/Fe3O4-OA magnetic targeted nanoparticle as well as preparation method and application thereof

A technology of targeting nanoparticles and magnetic nanoparticles, which is applied in the field of pharmaceutical chemical synthesis and preparation, can solve the problems of reducing side effects and inability to concentrate drugs, and achieves the effects of reducing toxic side effects, enhancing targeting selectivity, and improving curative effect.

Inactive Publication Date: 2011-11-02
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The biggest problem in general cancer treatment is that it is impossible to enrich the drug on cancer cells in a targeted manner, so that the side effects cannot be minimized

Method used

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  • Co-immobilized TNF-a/IFN-g/DOX/FA/Fe3O4-OA magnetic targeted nanoparticle as well as preparation method and application thereof
  • Co-immobilized TNF-a/IFN-g/DOX/FA/Fe3O4-OA magnetic targeted nanoparticle as well as preparation method and application thereof
  • Co-immobilized TNF-a/IFN-g/DOX/FA/Fe3O4-OA magnetic targeted nanoparticle as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Fix TNF-α / IFN-γ / Dox / FA / Fe 3 O 4 -OA Magnetic targeting nanoparticles for preparation

[0041] 1 Preparation of departure TNF-α / IFN-γ / DOX / FA

[0042] Use 50 μL of PBS (-) solution to dissolve 50 μg of TNF-α (tumor necrosis factor-α), IFN-γ (interferon-γ), DOXORUBICIN, DOX (moldin), folic acid, FA (folic acid)After adjusting the required concentration 1ng / μL, use a disposable filter membrane for filtration sterilization and packaging.

[0043] Preparation and representation of light activity TNF-α / IFN-γ / DOX / FA

[0044] 2.1 Preparation of light activity TNF-α / ifn-γ

[0045] Add 50 μg of TNF-α and IFN-γ to 25 μl of 25 μl of 768 μg N-Amberamide D μF / PBS (pH7.4, the volume ratio is 4: 1) solution.The stirring reaction under the condition of avoiding light) is 48h.After the synthesis is over, use the ultrafiltration of centrifugal tube (μlipore μolecut Ⅱ, 10KNA). At a speed of 4000rpm / min, centrifugal 30min is purified by purified nitrogen benzene derivatives.And adjust ...

Embodiment 2

[0057] Example 2 Pentillery Test

[0058] 1. Cultivation of human cervical cancer cells (Hela cell line)

[0059] Use RPMI 1640 medium (including 10%new calf serum, 0.03 μg / μL penicillin, 0.05 μg / μL chaincin, pH = 7.2-7.4), at 37 ° C, 5%CO 2 Routine secondary training in the training box.

[0060] Construction of cervical cancer nude mice model

[0061] Choose BALB / C naked mice (Nudemice), 4 weeks of age, 11.5 ± 1.50 g, a total of 18, all females.Guangdong) 2008-0002.Rest in the barrier system (SPF) of the Animal Experimental Center of the North Campus of Sun Yat -sen University.After a week of adaptability of nude rats, in the corresponding experimental nude mouse right thigh to inoculate HELA cells 5 × 10 7 cell / μL, 0.2 μL / size.After 3 weeks, the nude rats were 100%, and the drug began to inject drugs. There were 6 groups of experiments, numbered A, B, C, D, E, and F.Group A is a negative control. There are 2 nude rats, without inoculation of HELA cells, and no injection of ...

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Abstract

The invention discloses a co-immobilized TNF-a / IFN-g / DOX / FA / Fe3O4-OA magnetic targeted nanoparticle as well as the preparation method and application thereof. In the invention, three high-efficiency anti-cancer medicines which are tumour necrosis factor-a, interferon-g and adriamycin as well as cancer cell targeted folic acid are connected with a photoactive group; the three photoactive tumour necrosis factor-a, interferon-g and adriamycin as well as the folic acid are connected to a nano-carrier via an amidation reaction by adopting a photochemical fixation method to obtain a magnetic nanometre compound particle with a multilayer structure and a plurality of functions. The co-immobilized TNF-a / IFN-g / DOX / FA / Fe3O4-OA magnetic targeted nanoparticle of the invention has a good effect to externally inhibit HeLa cell growth, and has no obvious toxic and side effects to normal tissues, which can be used for preparing a medicine treating tumour.

Description

[0001] A co-fixed TNF-A / IFN-G / Dox / FA / Fe 3 O 4 -OA magnetic targeted nanoparticles and their preparation methods and applications Technical field [0002] The present invention involves the field of pharmaceutical chemical synthesis preparation technology, and specifically involves a common fixed TNF-α / IFN-γ / DOX / FA / Fe 3 O 4 -OA magnetic targeted nanoparticles and their preparation methods and applications. Background technique [0003] Cercinoma of Cervix is a common malignant tumor that is second only to breast cancer in the worldwide of women worldwide.The incidence is much lower than breast cancer, endometrial cancer and ovarian cancer.It is a malignant tumor that usually occurs at the junction of the squamous epithelial cells that usually occurs in the cervical vulva or the pillar epithelial cells of the neck tubular endometrium.In the past 50 years, with the development of socioeconomic and health, the incidence of cervical cancer in developed countries has fallen sharply, but...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K47/48A61K47/02A61K47/22A61K38/21A61K38/19A61K31/704A61P35/00
Inventor 关燕清刘俊明郑哲
Owner SOUTH CHINA NORMAL UNIVERSITY
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