Environmentally-friendly abamectin ointment production process
A technology of abamectin and production technology, applied in the field of production technology of environment-friendly abamectin ointment, can solve problems such as farmland and plant pollution, achieve the effects of improving product purity, reducing production cost, and simplifying separation and purification steps
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Embodiment 1
[0012] A. Streptomyces avermanii, the abamectin-producing strain, was first naturally isolated in the strain room, coated with double-disc culture medium, and cultured at a constant temperature (28.0±0.5°C) for 7 days, and the single bacteria fell into the slope of the eggplant bottle at a constant temperature ( 28.0±0.5°C) for 10 days, and the slant strains with high titer were screened out by shaking flasks, and the spores or hyphae of the strains produced were inserted into the seed tank under sterile conditions. In the seed medium, After culturing for 45 hours under suitable culture conditions, when the concentration of the seed liquid reaches above 28%, the hyphae form a net and tend to form balls, and then transfer to a fermenter. In the fermentation medium, after culturing for 300 hours under suitable culture conditions, when the total sugar consumption is below 2%, and the reducing sugar consumption is below 0.8%, the hyphae have obvious autolysis phenomenon under the m...
Embodiment 2
[0036] A. Streptomyces avermanii, the abamectin-producing strain, was first naturally isolated in the strain room, coated with double-disc culture medium, and cultured at a constant temperature (28.0±0.5°C) for 7 days, and the single bacteria fell into the slope of the eggplant bottle at a constant temperature ( 28.0±0.5°C) for 10 days, and the slant strains with high titer were screened out by shaking flasks, and the spores or hyphae of the strains produced were inserted into the seed tank under sterile conditions. In the seed medium, After culturing for 45 hours under suitable culture conditions, when the concentration of the seed liquid reaches above 28%, the hyphae form a net and tend to form balls, and then transfer to a fermenter. In the fermentation medium, after culturing for 300 hours under suitable culture conditions, when the total sugar consumption is below 2%, and the reducing sugar consumption is below 0.8%, the hyphae have obvious autolysis phenomenon under the m...
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