Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection of HLA genotype

A genotype, HLA-B technology for rapid genetics

Active Publication Date: 2014-02-12
THE CHINESE UNIVERSITY OF HONG KONG +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, LAMP has not been developed for human HLA genotyping

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection of HLA genotype
  • Detection of HLA genotype
  • Detection of HLA genotype

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0062] Materials and methods:

[0063] Study Design and Samples

[0064] In order to study the feasibility and accuracy of detecting specific HLA genotypes by the new detection method using B*1502 as an example, in the first phase of the study, healthy blood donors were recruited anonymously and 50 DNA samples were obtained (B* 1502 positive 25 and B*1502 negative 25) and 200 frozen blood samples (B*1502 positive 30 and B*1502 negative 170). All of these 250 samples were typed in a previous HLA genetic association study by high-resolution SBT as a control and were thus known to be HLA-B genotyped. SBT was performed according to the IHWG technical manual (8), and the resulting sequences were analyzed using the SBT tool (Genome Diagnostics, the Netherlands). Carrier status of B*1502 was detected directly in DNA samples by the novel LAMP method, and in frozen blood samples after heat treatment without DNA extraction. To further validate the application of this new assay in a c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed is a rapid genetic method for detection of HLA genotype based on the loop-mediated isothermal amplification (LAMP) principles, and in particular, disclosed are a method and a kit for detection of HLA-B*1502 allele based on LAMP principles.

Description

field of invention [0001] The invention relates to a rapid genetics method for detecting HLA genotypes based on the principle of loop-mediated isothermal amplification (LAMP). Specifically, the present invention relates to a method or a kit for detecting HLA-B*1502 alleles based on the principle of LAMP. Background of the invention [0002] Human leukocyte antigen (HLA) is a cell surface molecule that is delicately involved in and plays an important role in the presentation of processed antigenic peptides for T cell activation. HLA is encoded by a gene complex on the short arm of chromosome 6, which constitutes one of the most important immune genetic systems in the body. The HLA gene complex is not only polygenic but also multiallelic, so a high diversity of different HLA proteins can be produced in different individuals. Polymorphisms in this mechanism have been shown to be associated with individual variation in susceptibility to immune-mediated / controlled diseases and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6881C12Q2600/106C12Q2600/156C12Q2600/16
Inventor 郑淑恒关国良吴香玲
Owner THE CHINESE UNIVERSITY OF HONG KONG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com