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Application of protein and proteome in preparation of liver cirrhosis diagnostic reagent

A diagnostic reagent, a technology for liver cirrhosis, applied in the application field of protein and proteome in liver cirrhosis diagnostic reagent

Inactive Publication Date: 2011-09-14
天津宝瑞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, its accuracy in predicting liver fibrosis was only 51%, proving to be lower than that of Fibrotest

Method used

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  • Application of protein and proteome in preparation of liver cirrhosis diagnostic reagent
  • Application of protein and proteome in preparation of liver cirrhosis diagnostic reagent
  • Application of protein and proteome in preparation of liver cirrhosis diagnostic reagent

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Embodiment Construction

[0022] The following examples are used to further illustrate the present invention. The following examples are illustrative and not restrictive. The following examples cannot be used to limit the protection scope of the present invention.

[0023] The technical scheme of the present invention is as follows:

[0024] 1. Normal F0, liver fibrosis F1, F2, F3, F4, 6 biopsies in each group were identified and graded by Bartz and Ludwig method;

[0025] 2. Serum protein is used to remove 12 high-abundance proteins through IgY12 Beckman system;

[0026] A) CyDye labeled protein-DIGE analysis-Decyder-statistical analysis-primary marker 1;

[0027] B)iTRAQ 8-labeled protein-----iTRAQ analysis---statistical analysis---primary marker 2;

[0028] 3. The primary selection markers 1 and 2 are statistically analyzed ---candidate markers;

[0029] 4. Establish ELISA for candidate markers and identify markers.

[0030] Specific steps are as follows:

[0031] Sample analysis: The procedures for collecting sa...

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Abstract

The invention relates to application of protein and proteome in preparation of a liver cirrhosis diagnostic reagent. The protein comprises Lumican, Tetranectin, Pro-platelet basic protein (PBP), Pigment epithelium-derived factor (PEDF), Insulin-like growth factor binding protein 3 (IGFbp-3), Sex hormone-binding globulin (SHBG), Thioredoxin and composition of parts in the liver cirrhosis diagnostic reagent. Multiple proteins related with health and disease states are identified and evaluated in large range by adopting a proteome technology, the screened biomarkers for identifying hepatitis and liver fibrosis are used for predicting the degree of hepatitis and liver fibrosis and can be used for preparing a kit for diagnosing liver cirrhosis and liver cirrhosis level, and the protein markers identify the sensitivity of liver cirrhosis and degree thereof; and by combining the optimal conditions of various protein markers, the sensitivity can reach 89 percent, the specificity reaches over 90 percent, and the sensitivity and the specificity are higher than those of any known detection method.

Description

Technical field [0001] This patent belongs to the field of detection reagents, especially the application of a protein and proteome in diagnostic reagents for liver cirrhosis. Background technique [0002] Liver cirrhosis (Liver cirrhosis) is a common chronic liver disease, which can cause liver damage from one or more causes, and the liver presents progressive, diffuse, and fibrotic lesions. The specific manifestations are diffuse degeneration and necrosis of liver cells, followed by fibrous tissue proliferation and nodular regeneration of liver cells. These three changes are repeated and interlaced. As a result, the structure of liver lobules and blood circulation are gradually remodeled, which makes the liver deformed and hardened. Lead to liver cirrhosis. There are no obvious symptoms in the early stage of the disease, and a series of portal hypertension and liver dysfunction appear in the later stage, until the upper gastrointestinal bleeding, hepatic encephalopathy and oth...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 杜勇杨靖
Owner 天津宝瑞生物技术有限公司
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