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Molecular label from high-quality variety Xinluzao No.24 and relevant with cotton fiber strength

A technology of cotton fiber and molecular markers, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of not considering breeding selection, lack of stability and reliability of QTLs, etc., and improve breeding efficiency Effect

Active Publication Date: 2011-09-14
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

However, some of these mapped QTLs lack stability and reliability, and some of them are not considered in combination with breeding selection in the selection of experimental materials, so it is difficult to apply to breeding practice

Method used

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  • Molecular label from high-quality variety Xinluzao No.24 and relevant with cotton fiber strength
  • Molecular label from high-quality variety Xinluzao No.24 and relevant with cotton fiber strength
  • Molecular label from high-quality variety Xinluzao No.24 and relevant with cotton fiber strength

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Embodiment Construction

[0025] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0026] The present invention is linked with the molecular marker of the main effect gene of cotton fiber strength, which is screened out by the following method:

[0027] (1) In the first group, a late-maturing, high-quality medium-long-staple cotton Xinluzao 24 was used as the male parent and a transgenic insect-resistant conventional variety Lumianyan 28 was used as the female parent to prepare a hybrid combination. At the end of the year, F 1 F 2 Seed, the next spring general (Lumianyan No. 28 × Xinluzao 24) F 2 238 individual plants were obtained through planting. Change all F 2 F 2:3 seed. Planting 238 F in the third year 2:3 Family Seed. 15 strains were randomly selected from each family. Samples were taken after the bolls were opened in the autumn of the second y...

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Abstract

The invention discloses a molecular label linked with a major gene of a high-strength cotton fiber, which is obtained by the following method: taking the Xinluzao No.24 as a male parent, taking Lunianyan No.28 and Ji cotton 516 as female parents; respectively constructing two segregation populations of F2 and F2:3; carrying out polymorphism screening between parents (Lunianyan 28 and Xinluzao 24)by utilizing an SSR (simple sequence repeat) primer; constructing a genetic linkage map by utilizing F2; carrying out two generation quantitative trait locus (QTL); selecting an SSR label linked withthe high-strength cotton fiber to analyze the DNA polymorphism of the parent Xinluzao 24 and the Ji cotton 516; and constructing the genetic linkage map and positioning QTL; the fiber strength major QTL (qFS-62) can be stably detected in two populations and generations, and is positioned between the label interval of DPL0757-DPL0920 of chromosome c7; a synergtic gene is from the parent Xinluzao No.24; and the linking label of qFS-6-2 is DPL0757130, DPL0852170 and DPL0920130. By utilizing the label provided by the invention, molecule label assisted selection is carried out, thus improving the selection efficiency of the high-strength cotton fibre.

Description

technical field [0001] The present invention relates to a kind of SSR (Simple Sequence Repeat, simple repeating sequence) molecular marker linked with the main effect gene of fiber strength of Xinluzao 24, a high-quality medium and long-staple cotton variety, which is used for molecular marker-assisted selection to improve cotton fiber quality. To improve the efficiency of selection and speed up the pace of breeding. Background technique [0002] Cotton is an important raw material for the textile industry. Cotton production plays an important role in the national economy. Before the 1980s, the production of cotton in my country was mainly focused on the increase of yield, and no attention was paid to the improvement of fiber quality. As a result, most of the main cotton varieties in my country had high yield and poor quality. Compared with the United States, my country's cotton fiber length has an advantage, but its fiber specific strength is slightly worse than that of t...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 袁友禄孔凡金李俊文龚举武石玉真刘爱英商海红龚万奎王涛
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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