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High-efficiency functional marker large-scale development and quick QTL (Quantitative Trait Loci) location candidate gene determination method

A technology of candidate genes and markers, which is applied in the field of molecular genetics and molecular breeding, can solve the problems of non-gene region marker identification bias, etc., and achieve the effects of saving manpower and material resources, high polymorphism, and simple operation

Inactive Publication Date: 2011-09-07
SOUTHWEST UNIVERSITY
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Problems solved by technology

Therefore, at present, molecular markers of target gene regions or regions closely linked to target genes are rarely used for diversity analysis to overcome the problem of identification bias of non-gene region markers

Method used

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Embodiment Construction

[0022] Development of Functional Markers and Identification of Candidate Genes in Brassica napus Anthesis Regulatory Genes

[0023] 1. Determine the target traits of the research: genes regulating flowering period in Brassica napus;

[0024] 2. Download the whole genome sequence of Arabidopsis: Enter the Arabidopsis website: http: / / www.arabidopsis.org / , click "sequence" in the "download" drop-down box, select the directory "whole chromosomes", and download after the pop-up page 5 chromosomes of Arabidopsis, and saved to the hard disk;

[0025]3. Download the Arabidopsis thaliana flowering regulation gene sequence: Enter the Arabidopsis website: http: / / www.arabidopsis.org / , click the "search" tab, select "Genes" in the drop-down menu, and after the pop-up page, " Under "Search by Name or Phenotype", select the corresponding options according to your needs. For example, you need to include "flowering period" in the gene name, so select the "Gene name" "contains" tab, enter "flo...

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Abstract

The invention relates to a high-efficiency functional marker large-scale development and quick QTL (Quantitative Trait Loci) location candidate gene determination method, belonging to the fields of molecular genetics and molecular breeding. The method comprises the following steps of: comparing the gene sequence of an known function of another species with the genome sequences of a researched species (target species) to find out a homologous gene sequence; and then extracting the homologous gene sequence of an known function of the target species and a sequence in a 50kb range at the upstream and the downstream of the homologous gene sequence, finding out SSR (Simple Sequence Repeat) loci, developing a functional SSR marker closely linked with a target trait, integrating the results of the QTL location, and finally quickly determining candidate genes in the region wherein the QTL are located. The SSR marker developed by the method disclosed by the invention has the advantages of high polymorphism, stable and reliable result, good repeatability, convenience for operation, low cost and the like and is quite suitable for quickly encrypting the marker and determining the candidate genes after OTL primary location, thereby realizing confirming a plurality of trait candidate genes in a large scale and eliminating time-consuming and labor-consuming defects of a traditional method of further encrypting the marker and finding the candidate genes after the OTL primary location.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics and molecular breeding, in particular to a method for large-scale development of efficient functional markers and rapid determination of candidate genes for QTL positioning, which is specially used for the development of functional markers of target genes, rapid QTL fine positioning and efficient determination of candidates Gene. Background technique [0002] As the second-generation PCR-based molecular marker technology, SSR has the characteristics of high polymorphism, stable and reliable results, good repeatability, and easy operation, and SSR markers cover the entire genome, mainly manifested as co-dominant inheritance. In addition, SSR analysis does not require high DNA quantity and purity. The development of SSRs in gene regions or regions closely linked to genes can inherit a series of advantages of SSR markers, and compared with other markers, it reflects the genetic law of tar...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 付东辉付鹰李加纳魏丽娟钱伟阎星颖
Owner SOUTHWEST UNIVERSITY
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