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Method for separating, domesticating and cultivating wild Pholiota squarrosa strain

A technology of bacterial strain separation and cultivation method, which is applied in the directions of botanical equipment and methods, fertilizer mixture, gardening, etc., and can solve problems such as failure to carry out artificial domestication and cultivation.

Inactive Publication Date: 2012-07-25
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the rust umbrella has such rich nutritional and health value, it has not been domesticated and cultivated so far.

Method used

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  • Method for separating, domesticating and cultivating wild Pholiota squarrosa strain
  • Method for separating, domesticating and cultivating wild Pholiota squarrosa strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Strain isolation:

[0022] a. Using conventional PDA medium, the tissue separation method is used to separate the bacterial block from the cap of the wild Cinnamon chinensis, and then take 5 pieces of bacterial blocks of about 1 square millimeter, inoculate them in 5 test tubes, and cultivate them at 25°C 25 days to obtain tawny mother species;

[0023] Liquid propagation of strains:

[0024] b, using shake flasks to cultivate and propagate mother species to obtain cultivars, the liquid propagation medium is: potato 100g / L, bran 15g / L, maltose 25g / L, yeast powder 3g / L, potassium dihydrogen phosphate 0.5g / L L, dipotassium hydrogen phosphate 1g / L and magnesium sulfate 0.5g / L, insert the mother seed of the test tube into the liquid propagation medium, the temperature is 25°C, and cultivate for 10 days to obtain the cultivar;

[0025] Cultivation medium formula and production:

[0026] c. Weigh 50.7% of sawdust, 25% of cottonseed hulls, 15% of bran, 6% of cornmeal, 3% of...

Embodiment 2

[0032] Strain isolation:

[0033] a. Using conventional PDA medium, use the tissue separation method to separate the bacterial block from the cap of the wild cymbidium rotundum, and then take 5 bacterial blocks of about 1 square millimeter and inoculate them in 5 test tubes at a temperature of 25°C Cultivate for 30 days to obtain the tawny mother species;

[0034] Liquid propagation of strains:

[0035] b, using shake flasks to cultivate and propagate mother species to obtain cultivars, the liquid propagation medium is: potato 150g / L, bran 12g / L, maltose 23g / L, yeast powder 3g / L, potassium dihydrogen phosphate 0.5g / L L, dipotassium hydrogen phosphate 1g / L and magnesium sulfate 0.5g / L, insert the mother seed of the test tube into the liquid medium, and cultivate it for 13 days to obtain the cultivar at a temperature of 25°C;

[0036] Cultivation medium formula and production:

[0037] c. Weigh 68.9% of sawdust, 15.5% of cottonseed hulls, 12.5% ​​of bran, 4% of cornmeal, 1% o...

Embodiment 3

[0043] Strain isolation:

[0044] a. Using conventional PDA medium, separate the mother species from the cap of the wild C. chinensis by tissue separation method, then take 5 pieces of bacteria blocks of about 1 square millimeter, and inoculate them in 5 test tubes respectively, and cultivate them at 25°C Obtain tawny mother species in 35 days;

[0045] Liquid propagation of strains:

[0046] b, using shake flasks to cultivate and propagate mother species to obtain cultivars, the liquid propagation medium is: potato 200g / L, bran 10g / L, maltose 20g / L, yeast powder 2g / L, potassium dihydrogen phosphate 0.5g / L L, dipotassium hydrogen phosphate 1g / L and magnesium sulfate 0.5g / L, insert the mother seed of the test tube into the liquid medium, and cultivate it for 15 days to obtain the cultivar at a temperature of 25°C;

[0047] Cultivation medium formula and production:

[0048] c. Weigh 59.8% of sawdust, 20% of cottonseed hulls, 13% of bran, 5% of cornmeal, 2% of gypsum, and 0.2...

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Abstract

The invention relates to a method for separating, domesticating and cultivating a wild Pholiota squarrosa strain. In the method, a new edible mushroom variety Pholiota squarrosa can be obtained by separating, proliferating, domesticating and cultivating the strain; and the wild Pholiota squarrosa can be manually domesticated and cultivated by using the method provided by the invention to provide an excellent new variety for the edible mushroom market. The cultivation method provided by the invention has the advantages of mushroom producing time of about one month and biological conversion of 30-40%. The Pholiota squarrosa obtained by the method provided by the invention has the advantages of light yellow or khaki color, yellowish-brown reflexed scales on a pileus and a stipe, delicious taste, anti-cancer and antitumor effects and wide popularity among people.

Description

technical field [0001] The invention belongs to the domestication and cultivation technology of edible fungus, and relates to the strain separation, multiplication, cultivation material and domestication method of the fungi. Background technique [0002] At present, there is a single variety of edible fungi in the domestic edible fungus market, mainly shiitake mushrooms, Agaricus bisporus, Asafoetida, Flammulina velutipes, Oyster mushrooms, Pleurotus eryngii, tea tree mushrooms, and Coprinus comatus. Along with the raising of people's living standard, require new multi-variety gradually, the development of new edible fungus variety becomes the needs of situation development. my country has abundant edible fungi resources, and what is currently being developed is only the tip of the iceberg of wild edible fungus resources. Cap and stipe have obvious recurved scales. The Japanese found that the extract of Umberella chinensis has free radical capture ability and tyrosinase ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 付振艳王晓军吴金平
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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