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Primers and probes for detecting genes associated with schizophrenia, bipolar affective disorders and major depression and kits and preparation methods thereof

A technology for bipolar disorder and schizophrenia, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/testing, etc., can solve problems such as high price, high cost, and long cycle

Inactive Publication Date: 2011-08-10
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TaqMan probes need to be customized from AB company, which takes a long time and is expensive
Although the results of sequencing technology are more reliable, but for the SNP typing of specific loci, this method is relatively expensive and takes a long time

Method used

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  • Primers and probes for detecting genes associated with schizophrenia, bipolar affective disorders and major depression and kits and preparation methods thereof
  • Primers and probes for detecting genes associated with schizophrenia, bipolar affective disorders and major depression and kits and preparation methods thereof
  • Primers and probes for detecting genes associated with schizophrenia, bipolar affective disorders and major depression and kits and preparation methods thereof

Examples

Experimental program
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Embodiment 1

[0031] 1. Blood Sample Collection and Total DNA Extraction

[0032] The peripheral blood of volunteers was collected to prepare total DNA. DNA was extracted directly using Qiagen's DNA extraction kit, and the extraction method was carried out according to the instructions of the kit. After extraction, use NanoDrop to detect the concentration of each DNA sample, then adjust each sample to 10 ng / μL, and store it at -20 degrees for later use.

[0033] 2. PCR reaction

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PUM

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Abstract

The invention discloses a kit for detecting genes associated with schizophrenia, bipolar affective disorders and major depression, which belongs to the field of biotechnology, and consists of a polymerase chain reaction (PCR) reagent group and a ligase detection reaction (LDR) reagent group, wherein the PCR reagent group comprises buffer solution, deoxyribonucleotide triphosphate (dNTP) mixed solution, Taq DNA polymerase, pure water, an upstream primer represented by SEQ ID No.1 and a downstream primer represented by SEQ ID No.2; and the LDR reagent group comprises buffer solution, dNTP mixed solution Taq DNA polymerase, pure water, a probe represented by SEQ ID No.3, a probe represented by SEQ ID No.4 and a probe represented by SEQ ID No.5. In the invention, the operation is simple and convenient, the cost is low, and the kit is developed for bipolar affective disorders. The result is reliable, the stability is high and the sensitivity is high.

Description

technical field [0001] The present invention relates to a kit in the field of molecular biotechnology and a preparation method thereof, in particular to a primer, a probe and a kit thereof for the detection of genes associated with schizophrenia, bipolar disorder and major depression and preparation method. Background technique [0002] Ligase can connect two oligonucleotide fragments that are completely complementary to the target sequence to form a longer oligonucleotide probe. If they are not completely complementary, then the ligation reaction will not take place and no longer oligonucleotide probes will be produced. According to this principle, the genotype of the SNP site can be detected by designing oligonucleotide probes of different lengths according to the genotype of the SNP site. According to the length classification of the final product, the genotype of the SNP site can be determined, which is the ligase detection reaction (Ligase Detection Reaction). [000...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 师咏勇李俊燕贺林陈培华
Owner SHANGHAI JIAO TONG UNIV
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