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Method for preparing high-purity geniposide

A high-purity technology of geniposide, applied in the field of medicine, can solve the problem of low purity of geniposide, and achieve the effect of simple preparation process

Inactive Publication Date: 2011-08-10
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that in the current research, the macroporous resin adsorption technology is used to purify and prepare geniposide, but the purity of geniposide is relatively low when the macroporous resin adsorption technology is used alone, and it needs to be further refined in combination with other refining processes. Research

Method used

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  • Method for preparing high-purity geniposide
  • Method for preparing high-purity geniposide
  • Method for preparing high-purity geniposide

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Preparation of gardenia extract: take 1Kg of dried gardenia medicinal material (the content of geniposide is 4.23%), soak in water for 0.5h, decoct 3 times, add 10 times of water each time, each time for 1.5h, filter, Combine the filtrates, concentrate the filtrates to 1:1 (each ml contains 1 g of crude drug), add 95% ethanol until the alcohol content of the concentrated solution is 70%, place it in refrigeration for 48 hours, centrifuge, collect the supernatant, and recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste, and concentrated to a concentrated solution containing 0.5g of crude drug per 1ml of the extract, the volume of the extract is 2L, i.e. the gardenia extract.

[0028]2. Purification of geniposide with macroporous resin: Take 4.4Kg of the pretreated macroporous resin and put it into a glass column with a little absorbent cotton. Put a little absorbent cotton on the resin column, the volume of the macroporous resi...

Embodiment 2

[0033] 1. Preparation of gardenia extract: take 1Kg of dried gardenia medicinal material (the content of geniposide is 4.23%), soak in water for 0.5h, decoct 3 times, add 10 times of water each time, each time for 1.5h, filter, Combine the filtrates, concentrate the filtrates to 1:1 (each ml contains 1 g of crude drug), add 95% ethanol until the alcohol content of the concentrated solution is 70%, place it in refrigeration for 48 hours, centrifuge, collect the supernatant, and recover the ethanol from the filtrate under reduced pressure until there is no alcohol taste, and concentrated to a concentrated solution containing 0.5g of crude drug per 1ml of the extract, the volume of the extract is 2L, i.e. the gardenia extract.

[0034] 2. Purification of geniposide with macroporous resin: Take 4.4Kg of the pretreated macroporous resin and put it into a glass column with a little absorbent cotton. Put a little absorbent cotton on the resin column, the volume of the macroporous res...

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Abstract

The invention discloses a method for preparing high-purity geniposide by extracting and purifying a cape jasmine fruit. The method comprises the following steps of: preparing a cape jasmine extract by adopting a water extraction and alcohol precipitation method; preparing a geniposide rough extract by purifying through adopting a macroporous resin adsorption eluting technology; and refining the geniposide by adopting an aluminum oxide column chromatography technology or n-butyl alcohol extraction technology to obtain the high-purity geniposide. The preparation method disclosed by the invention has the advantages of simplicity in operation, low cost, high product purity, high yield and no environmental pollution and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for extracting and purifying high-purity geniposide from gardenia fruit. Background technique [0002] Gardenia is the dry ripe fruit of Gardenia jasminoides Ellis. Harvest when the fruit is ripe and red-yellow in September-November. Remove fruit stalks and impurities, steam to steam or put in boiling water for a while, take out, and dry [State Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China (2005 Edition Part One) [S]. Beijing: Chemical Industry Press, 2005: 173]. Bitter and cold in nature and flavor, enters the heart, liver, lungs, stomach, and triple burner, and has the effects of purging fire and relieving restlessness, clearing heat and dampness, cooling blood and detoxification, reducing swelling and relieving pain. [0003] Gardenia contains a large amount of iridoid glycosides [Wang Gangli, Chen Dechang, Zhao Shujie. Research progress ...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/04
Inventor 张彤丁越李婷尹胜利黄孝春
Owner SHANGHAI UNIV OF T C M
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