Miniature capillary array device for isoelectric focusing electrophoresis

Abstract

The invention relates to a miniature capillary array device for isoelectric focusing electrophoresis (IEF) in the technical field of biochemical engineering. The device consists of an array type electrophoresis tank and miniature capillaries, wherein the array type electrophoresis tank has two symmetrical parts; a groove array is carved on the array type electrophoresis tank; and the miniature capillaries are arranged in the groove array. One pair or multiple pairs of the array devices of the invention are selected to perform IEF simultaneously according to need and the separation flux of theprotein sample can be from dozens of tubes to hundreds of tubes. The diameter and length of the miniature capillaries in the device are shorter, thus the IEF time and cost can be effectively reduced.

Description

technical field [0001] The present invention relates to an array device in the field of biochemical technology, in particular to a microcapillary array device for isoelectric focusing electrophoresis (Isoelectric Focusing Electrophoresis, IEF). Background technique [0002] Isoelectric focusing electrophoresis is the most effective and basic technology in protein separation. Its method is to separate proteins and polypeptides in solution according to their own isoelectric points under the action of an applied electric field force. Since the 1950s, IEF has been widely used in the separation and analysis of proteins, peptides and other substances. As in the prior art literature: [0003] 1. Application number: 02108371.1, name: An electrophoresis focusing and concentrating device. The device involved in this technology is equipped with two electrolytic cells, which are connected by tubes and separated by solid ion conductors. It is designed using the principle of aggregation...

AI Technical Summary

Problems solved by technology

However, the traditional IEF device has too few separation channels, which largely leads to the low flux of IEF separation proteins.

However, if multiple sets of IEF traditional devices are used, the preparation work is complicated and the cost of the experiment increases sharply, so the traditional devices are difficult to be used for high-throughput separation and analysis in IEF

[0008] Second, the separation time is long and the resolution is low

[0009] Third, poor reproducibility

In the traditional IEF method, the large tubes are injected one by one (Righetti, P.G., Bossi, A. Analytica Chimica Acta, 372, 1), the preparation steps are cumbersome and the gel standards are different between tubes due to different injection times , so that when IEF is performed, the pH gradient formed is not uniform, and the final separation position of the same protein bands is deviated, so it is necessary to improve the traditional large tube separation

[0010] Fourth, poor stability

Therefore, the poor linear relationship of pH gradient and cathode drift seriously affect the stability of IEF.

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