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Method for manufacturing electrochemical acetylcholinesterase biological sensor

An acetylcholinesterase and biosensor technology, applied in the field of sensors, can solve the problems of high missed detection ratio, low recovery rate, poor repeatability, etc., and achieve the effects of preventing interference, simple operation and simple structure

Active Publication Date: 2011-06-22
SHANDONG UNIV OF TECH
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Problems solved by technology

The principle of spectrophotometry is based on the change of absorbance for detection, but a large number of pigments in vegetables and fruits will have a great impact on spectrophotometry, resulting in inaccurate detection results
However, the above method has disadvantages such as low recovery rate, high proportion of false detection and missed detection, poor repeatability, and difficulty in meeting the requirements of low residue and quantitative detection.

Method used

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  • Method for manufacturing electrochemical acetylcholinesterase biological sensor
  • Method for manufacturing electrochemical acetylcholinesterase biological sensor
  • Method for manufacturing electrochemical acetylcholinesterase biological sensor

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Embodiment Construction

[0029] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific diagrams.

[0030] refer to figure 1 , figure 2 , image 3 A preparation method of an electrochemical acetylcholinesterase biosensor comprises the following steps: the first step, the manufacturing process of the enzyme film, the second step, the modification process of the glassy carbon electrode, and the third step, the fixing process of the enzyme film. In the second step, the glassy carbon electrode modification process uses functionalized multi-walled carbon to modify the glassy carbon electrode 4, including the following steps: Step 21, functionalization of multi-walled carbon nanotubes: weigh 10 mg of multi-walled carbon nanotubes Tube 5, put into a 100mL beaker, measure 40mL concentrated HNO 3 and H 2 SO 4 The mixed solution (V:V=1:3) was added int...

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Abstract

The invention relates to the technical field of sensors, in particular to an acetylcholinesterase biological sensor. A method for manufacturing an electrochemical acetylcholinesterase biological sensor comprises the following steps of: manufacturing an enzyme membrane; modifying a glassy carbon electrode by using functionalized multi-wall carbon, namely functionalizing a multi-wall carbon nanotube, cleaning, testing and modifying; and fixing the enzyme membrane. Due to the technical scheme, the acetylcholinesterase biological sensor manufactured by the method has the advantages of simple structure, high sensitivity, repeated detection and the like.

Description

technical field [0001] The invention relates to the technical field of sensors, in particular to an acetylcholinesterase biosensor. Background technique [0002] With the rapid and stable growth of my country's economy and the continuous improvement of people's living standards, the requirements for food consumption are also getting higher and higher. Returning to nature and consuming pollution-free food have become the consumption trend and market trend in the new era. However, environmental pollution poses a great threat to the hygienic quality of agricultural products, and food poisoning incidents have been reported continuously, which has aroused widespread concern. [0003] In order to ensure people's food safety, many agricultural product wholesale markets, hotels, restaurants and other places use testing equipment to detect pesticide residues in agricultural products. At present, the rapid detection methods of pesticide residues in my country are enzyme inhibition t...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/36
Inventor 孙霞王相友刘君峰赵文苹杜淑媛
Owner SHANDONG UNIV OF TECH
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