Broccoli Ogu cytoplasm quick identification kit and detection method thereof

Abstract

The invention discloses a broccoli Ogu cytoplasm quick identification kit and a detection method thereof. The kit comprises a reagent A and a reagent B, wherein the reagent A contains a PCR (Polymerase Chain Reaction) buffer solution, Taq polymerase, dNTP and an Oligo-dT primer, and the reagent B is standard DL-2000DNA Ladder. The detection method comprises the following steps of: DNA extraction of a sample; (1) genome DNA extraction of the sample; (2) PCR amplification reaction; (3) agarose gel electrophoresis detection by which whether the sample is Ogu cytoplasm or not is determined according to the existence / absence of 392bp specific amplification bands. By using the broccoli Ogu cytoplasm quick identification kit, the steps from DNA extraction to the obtaining of detection results can be finished only in several hours, which is very convenient and quick; and by the broccoli Ogu cytoplasm quick identification kit, screening and identifying can be finished in seedling stage, thereby greatly shortening identification cycle. The broccoli Ogu cytoplasm quick identification kit has very important value in broccoli breeding practices.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid identification kit for broccoli Ogu cytoplasm, which can detect broccoli Ogu cytoplasm and is suitable for rapid identification and molecular marker-assisted selection of the cytoplasm. Background technique [0002] Male sterility is a common natural phenomenon in plants, which means that due to various genetic or physiological reasons, the female organs of plants are normal, but the male organs are abnormal, unable to produce pollen or the pollen is aborted and cannot be pollinated normally, resulting in Self-crossing is not fruitful, only outcrossing is fruitful. Male sterility exists widely in the plant kingdom, and it has been found in many varieties or interspecific hybrids so far. [0003] Studies have shown that male sterility is mainly divided into two categories: nuclear male sterility (Genic male sterility, GMS) and cytoplasmic male sterility (abbreviated as CMS). ...

AI Technical Summary

Problems solved by technology

[0006] Traditional CMS identification and classification methods such as tester lines, cytological markers, protein markers, isoenzyme identification, etc. have disadvantages such as long identification period, time-consuming and labor-consuming, and complicated operation, and often cannot accurately identify unidentified species. Specific type of cytoplasm

At present, after searching, there is no rapid identification kit for broccoli Ogu cytoplasm at home and abroad.

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