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Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis

A technology of dehydrocostolactone and angiogenesis, which is applied in drug combination, cardiovascular system diseases, antineoplastic drugs, etc.

Inactive Publication Date: 2011-05-18
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the application of dehydrocressin in inhibiting angiogenesis.

Method used

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  • Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis
  • Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis
  • Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Dehydrocressin inhibits the proliferation of human umbilical vein endothelial cells (HUVEC)

[0033] The proliferation of HUVEC was detected by CCK-8 method. CCK-8 reagent contains WST–8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene) -2H-tetrazolium monosodium salt), which is reduced by the dehydrogenase in the mitochondria of the cell under the action of the electron carrier 1-Methoxy PMS (1-methoxy-5-methylphenazine dimethyl sulfate) to have Highly water-soluble yellow formazan (Formazan) product. The amount of formazan produced is directly proportional to the number of viable cells. The light absorption value was measured at a wavelength of 450 nm by an enzyme-linked immunosorbent detector, which can indirectly reflect the number of living cells. The effect of drugs on the proliferation of HUVCE can be evaluated by this method.

[0034] Methods: HUVEC cells were seeded in 96-well plates and cultured. After the cell growt...

Embodiment 2

[0038] Example 2 Dehydrocressin inhibits the migration of human umbilical vein endothelial cells (HUVEC)

[0039] Transwell method was used to detect HUVEC migration. Transwell is an experimental technology. The main material of this technology is the Transwell chamber (chamber). The bottom layer of the chamber has a layer of permeable membrane (polycarbonate membrane (polycarbonate membrane) is commonly used, and this membrane has pores. Micropores with a size of 0.1-12.0 μm. In the experiment, the cells are placed on the small chamber. Under the action of the chemokines or growth factors contained in the small chamber, the cells pass through the polycarbonate membrane, thereby performing co-culture, cell chemotaxis, cell Migration, cell invasion and many other studies.

[0040] Method: Add 600 μL of VEGF containing 20 ng / ml to the lower chamber 165 HUVEC culture medium. Added HUVEC containing different concentrations of dehydrocressin in the upper chamber (the cell densit...

Embodiment 3

[0042] Example 3 Dehydrocressin inhibits HUVEC tubule formation experiment

[0043] The tubule formation of HUVEC was detected by Matrigel gel. Artificial basement membrane Matrigel is a basement membrane component extracted from mouse EHS sarcoma. It can spontaneously form a gel-like artificial basement membrane at 37 °C, and has the biological function of a natural basement membrane. Human umbilical vein endothelial cells can adhere to form tubes on Matrigel glue, which can be used to study the effect of drugs on the tube formation of endothelial cells.

[0044] Method: 50 μl of Matrigel gel solution was added to a pre-cooled 96-well culture plate, and then placed in a 37 °C incubator for 1 h to solidify the gel. HUVEC (cell density 1×10 5 / ml) were added to the 96-well plate covered with Matrigel, placed in CO 2 Incubate for 10 h in the cell culture incubator. The group without dehydrocrestinolide was used as the control. After 10 h, blood vessel formation was observed...

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PUM

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Abstract

The invention relates to application of dehydrocostuslactone to preparing a drug for inhibiting angiogenesis. The invention has the advantages of providing the novel application of the dehydrocostuslactone, finding the anti-angiogenesis of the dehydrocostuslactone for the first time, finding that the dehydrocostuslactone can effectively inhibit the multiplication, the migration and the canaliculization of vascular endothelial cells for the first time, showing that the dehydrocostuslactone has the activity of the anti-angiogenesis and can be used as an angiogenesis inhibitor as well as can be used as the angiogenesis inhibitor used for preparing the angiogenesis drug and treating vascularization dependent and vascularization relevant diseases of tumors, arthritis, parapsoriasis guttata, eye disorders, atherosclerosis and the like.

Description

technical field [0001] The invention relates to the application of a Chinese herbal medicine monomer, in particular to the application of a Chinese herbal medicine monomer dehydrocressin in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis is a complex process involving multiple molecules from multiple cells, including endothelial cell secretion of proteases to degrade basement membrane, proliferation and migration of endothelial cells, formation of new capillary luminal structures, and recruitment of pericytes to stabilize newly formed capillaries The mature vascular network is formed through the remodeling and expansion of primitive blood vessels. [0003] The dynamic balance between angiogenesis-inhibiting molecules and angiogenesis-promoting molecules is considered to be the "switch" that regulates angiogenesis. In most cases, the two are in balance, and the angiogenesis mechanism is turned off; when the balance of the two ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P35/00A61P35/02A61P17/06A61P19/02A61P9/10A61P27/02
Inventor 陈红专方超陆琴赵梅於得红白帆王超
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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