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Plant stress resistance related protein GmSIK1, coding gene thereof and application thereof

A technology of plant stress tolerance and encoding genes, which is applied to soybean stress tolerance-related protein GmSIK1 and its encoding genes and applications, and can solve the problems of lack of data and late understanding of plant receptor kinases

Inactive Publication Date: 2011-05-04
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Understanding the transmission process of stress signals in plants is the key to understanding and regulating plant stress tolerance, and protein kinases, especially receptor kinases, play a key role in the signal transmission process, but due to the understanding of plant receptor kinases Late, information about its mechanism of action is still quite lacking

Method used

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  • Plant stress resistance related protein GmSIK1, coding gene thereof and application thereof
  • Plant stress resistance related protein GmSIK1, coding gene thereof and application thereof
  • Plant stress resistance related protein GmSIK1, coding gene thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077]Example 1, Screening of Soybean Stress Tolerance-Related Receptor Kinase Gene GmSIK1 and Cloning of Its cDNA

[0078] 1. Screening of GmSIK1

[0079] The completion of soybean genome sequencing provides favorable conditions for the discovery of new stress-resistant genes. According to the 30,000 EST sequences determined by the inventors and the more than 280,000 sequences downloaded from GenBank, EST clustering was performed together to obtain 56,147 single genes (unigenes), including 32,278 contigs (Contigs) and 23,869 single-copy sequences ( singleton). On the basis of the existing single gene (unigenes) annotation of Arabidopsis, the function of soybean single gene (unigenes) was classified and annotated. According to the alignment of known soybean receptor kinase sequences with soybean unigenes, 486 soybean receptor kinase candidate genes were obtained. 338 gene fragments with relatively complete sequences were selected to design RT-PCR primers, and their response...

Embodiment 2

[0103] Example 2, the expression characteristics of GmSIK1 in different tissues and organs

[0104] RNA was extracted from the roots, stems, and leaves of two-week-old soybean Nannong 1138-2 seedlings, and the field soybean Nannong 1138-2 flowers and pods, and the expression level of GmSIK1 was analyzed by RT-PCR. The tubulin gene was used as a control, and the primers were: 5'-AAC CTC CTC CTC ATC GTA CT-3' (SEQ ID NO: 9) and 5'-GAC AGC ATC AGC CATGTT CA-3' (SEQ ID NO: 10).

[0105] The expression characteristics of GmSIK1 gene in different tissues and organs are shown in image 3 . Among them, 1: root; 2: stem; 3: flower; 4: leaf; 5: pod.

[0106] The results showed that the expression of GmSIK1 was strong in leaves and weak in other tissues and organs.

Embodiment 3

[0107] Example 3, Expression Characteristics of Soybean GmSIK1 Gene under Different Adversity Stresses

[0108] Sow soybean Nannong 1138-2[Glycine max(L.)Merr] in flower pots filled with vermiculite, grow for 2 weeks (grow 5-6 true leaves), and then take the seedlings to undergo the following stress treatments:

[0109] 1) Abscisic acid (ABA) treatment: the roots were completely immersed in 100 μM ABA solution; 2) Salicylic acid (SA) treatment: the leaves of the seedlings were sprayed with 2 mM salicylic acid; 3) Drought stress treatment: the soybean seedlings were placed at room temperature Drought in the air; 4) Low temperature (4°C) stress treatment: completely immerse the roots in the aqueous solution, which is kept at 4°C; 5) Salt stress treatment: completely immerse the roots in the 200mM NaCl solution. 6) Control: The roots were completely immersed in the aqueous solution, and the aqueous solution was kept at room temperature.

[0110] After the above-mentioned treatme...

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Abstract

The invention discloses a plant stress resistance related protein GmSIK1, a coding gene thereof and application thereof. The protein is a protein (a) or a protein (b), wherein the protein (a) has amino acid sequences shown as a sequence 1 in a sequence table; and the protein (b) has an amino acid residue sequence which is derived from the sequence 1 by substituting and / or losing and / or adding one or more amino acid residues and is related with plant stress resistance. The coding gene of the protein is introduced into plant cells, and a transgenic plant with enhanced stress resistance can be obtained. The protein and the coding gene thereof have important significance for culturing stress-resistance plant varieties, particularly culturing plant varieties resisting abiotic intimidation such as draught and / or salt so as to improve crop yield.

Description

technical field [0001] The invention relates to a plant stress tolerance-related protein GmSIK1 and its encoding gene and application, in particular to the stress tolerance-related protein GmSIK1 derived from soybean and its encoding gene and application. Background technique [0002] The growth and development of plants are closely related to the external environment. Plants will experience various adversity stresses at each growth and development stage, including biotic stress and abiotic stress. Abiotic stresses such as drought, salinity, and low temperature are important factors that restrict plant growth and reduce crop yield and quality. In agricultural production, the loss of crop yield caused by abiotic stress reduces the average yield of major crops worldwide by more than 50%. After a long evolutionary process, plants have developed mechanisms to adapt to different adversities. One of the important mechanisms is to induce the expression of related genes. Genes in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/63C12N1/00C12N5/10C12N15/11C12N15/82
CPCC07K14/415C12N15/8273
Inventor 陈受宜张劲松刘鹏何锶洁张万科林晴
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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