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Application of Edible Vegetable Oil in Improving Poria Triterpene Content in Poria Mycelia

A technology for edible vegetable oil and Poria cocos triterpenes, which is applied to microorganism-based methods, uses added compounds to stimulate growth, fungi, etc., can solve problems such as potential safety hazards and inducer residues, and achieves increased intracellular content of Poria cocos triterpenes. Effect

Active Publication Date: 2022-04-05
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above defects or improvement needs of the prior art, the present invention provides an application of edible vegetable oil in increasing the content of Poria cocos triterpenoids in the mycelia of Poria cocos, the purpose of which is to induce liquid fermentation by using edible vegetable oil with food safety level The inducer that increases the content of Poria cocos triterpenes in Poria cocos mycelium can be added at a low dose to achieve an obvious effect of inducing the expression of Poria cocos triterpenes, thereby solving the existing inducer for increasing the content of Poria cocos triterpenes in Poria cocos mycelia Residues, technical problems with potential safety hazards

Method used

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  • Application of Edible Vegetable Oil in Improving Poria Triterpene Content in Poria Mycelia
  • Application of Edible Vegetable Oil in Improving Poria Triterpene Content in Poria Mycelia
  • Application of Edible Vegetable Oil in Improving Poria Triterpene Content in Poria Mycelia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Poria cocos liquid strain preparation:

[0041](1) Activate the Poria cocos mycelium with PDA liquid medium, at a temperature of 28-30°C, at a speed of 120-150r / min, and activate for 7 days.

[0042] (2) Inoculate 10% (v:v) of the Poria cocos mycelium in (1) into the Poria cocos seed culture medium, and cultivate for 5 days at a temperature of 28-30° C. and a rotational speed of 120-150 r / min.

[0043] Growth Stage Culture:

[0044] Inoculate the Poria cocos liquid strain (2) in the CYM medium, the inoculation amount of the seed solution is 1%, at a temperature of 28-30°C, a rotating speed of 120-150r / min, cultivate for 3 days, and carry out the first-stage fermentation to make the Poria cocos hyphae rapid growth and reproduction.

[0045] Induction culture stage:

[0046] After the fermentation of the growth stage, under aseptic conditions, 1% (v / v) of peanut oil, rapeseed oil, palm oil, soybean oil, olive oil, and coix seed oil were added as treatment groups (inocu...

Embodiment 2

[0051] Poria cocos liquid strain preparation:

[0052] (1) Activate the Poria cocos mycelium with PDA liquid medium, at a temperature of 28-30°C, at a speed of 120-150r / min, and activate for 7 days.

[0053] (2) Inoculate 10% (v:v) of the Poria cocos mycelium in (1) into the Poria cocos seed culture medium, and cultivate for 5 days at a temperature of 28-30° C. and a rotational speed of 120-150 r / min.

[0054] Growth Stage Culture:

[0055] Inoculate Poria cocos liquid strains into CYM medium, the inoculum amount of seed solution is 1%, and cultivate for 3 days at a temperature of 28-30°C and a rotation speed of 120-150r / min, and carry out fermentation in the growth stage, so that the Poria cocos mycelia can grow and reproduce rapidly .

[0056] Induction phase culture:

[0057] After the fermentation of the growth stage, olive oil with a final concentration of 1%, 2%, 3%, and 4% was added to the fermentation broth under sterile conditions, and the second stage of fermentat...

Embodiment 3

[0062] Poria cocos liquid strain preparation:

[0063] (1) Activate Poria cocos mycelium with PDA liquid medium, at a temperature of 28-30° C., and a rotation speed of 120-150 r / min, for 7 days.

[0064] (2) Inoculate 10% (v:v) of the Poria cocos mycelium in (1) into the Poria cocos seed culture medium, and cultivate for 5 days at a temperature of 28-30° C. and a rotational speed of 120-150 r / min.

[0065] Growth Stage Culture:

[0066] Inoculate the Poria cocos liquid strain (2) in the CYM medium, the inoculation amount of the seed solution is 1%, at a temperature of 28-30° C., and a rotating speed of 120-150r / min, cultivate for 3 days, and carry out the first-stage fermentation to make the Poria cocos mycelia Rapid growth and reproduction.

[0067] Induction phase culture:

[0068] After the fermentation of the growth stage, under aseptic conditions, the 0h, 24h, 48h, 72h, and 96h of the liquid fermentation were taken as the time points of adding 2% olive oil. Continue t...

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Abstract

The invention discloses an application of edible vegetable oil, which is characterized in that it is used to increase the content of Poria cocos triterpenes in the cells of Poria cocos mycelia. The invention adds edible vegetable oil to the culture medium of the fully grown Poria cocos mycelium, which can significantly increase the content of the poria cocos triterpene in the cells of the poria cocos mycelium. The preferred scheme can even increase the content of Poria cocos triterpenes to more than 4 times. Edible vegetable oil is safe to eat, and a small amount can induce the synthesis of triterpenes. The invention uses the edible vegetable oil as the inducer to realize the high-level expression of the triterpene content in the poria cocos.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to the application of edible vegetable oil in increasing the content of Poria cocos triterpenes in the cells of Poria cocos mycelia. Background technique [0002] Poria cocos (Poria cocos (Schw.) Wolf) is a large medicinal fungus belonging to the phylum Fungi, Basidiomycetes, Polyporaceae, Polyporaceae, and Poria cocos (Poria cocos), which has been used in my country for thousands of years. Modern science has confirmed that the chemical components of Poria cocos include polysaccharides, triterpenoids, sterols, alkaloid amino acids, etc., and one of the most important medicinal components is Poria cocos triterpenoids, which have anti-tumor, liver protection, regulating immunity etc. Increasing the content of triterpenoids in Poria cocos plays an important role in the wide application of Poria cocos. [0003] Current studies have shown that methyl jasmonate, acetic acid,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/14C12P33/00C12R1/645
CPCC12N1/38C12N1/14C12P33/00
Inventor 胡学博魏茹丹孟令会
Owner HUAZHONG AGRI UNIV
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