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Method for screening and remediation of petroleum-contaminated soil bioremediation agent

A technology of oil pollution and soil biology, applied in the field of microbial remediation of oil-contaminated soil, can solve the problems that ordinary microorganisms cannot use high-salinity oil-contaminated soil for biological treatment, and achieve high repair effect

Inactive Publication Date: 2011-04-20
BEIHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of the problems existing in the prior art, in order to solve the inhibitory effect of high salinity in oil-contaminated soil on microorganisms, ordinary microorganisms cannot be used for biological treatment of high-salinity oil-contaminated soil, the present invention provides a bioremediation bacterium for oil-contaminated soil The screening and remediation method of the agent, the bioremediation bacterial agent screened by the screening and remediation method can be applied to the biodegradation of oil-contaminated soil with a salt content of 10-20g / Kg; the bioremediation bacteria of oil-contaminated soil strengthened by halophilic bacteria The agent screening and remediation method has high practical value and can be widely used in bioremediation fields such as oil-contaminated soil, coastal beaches, and oil production wastewater.

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  • Method for screening and remediation of petroleum-contaminated soil bioremediation agent

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Embodiment Construction

[0052] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0053] The screening and restoration method of a kind of bioremediation bacterium of petroleum-contaminated soil that the present invention proposes specifically comprises the following steps:

[0054] Step 1: Collection of sources of oil-degrading indigenous bacteria and halophilic bacteria;

[0055] The oil-contaminated soil at the discharge outlet of oilfield wastewater was used as the source of oil-degrading indigenous bacteria; the oil production wastewater and activated sludge of sewage treatment plant at the outlet of oilfield wastewater treatment plant were used as the source of halophilic bacteria.

[0056] Step 2: Screening of indigenous oil-degrading bacteria, such as figure 2 shown;

[0057] (1) Take 2g of the soil samples collected from 9 sampling points, add them to 9 Erlenmeyer flasks (containing glass beads) filled with 100mL steril...

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Abstract

The invention provides a method for the screening and remediation of a petroleum-contaminated soil bioremediation agent, which specifically comprises the following six steps of: collecting a petroleum degradation autochtonous bacterium source and a halophilic bacterium source, screening petroleum degradation autochtonous bacteria, screening halophilic bacteria, screening the petroleum degradation halophilic bacteria, screening a high-efficiency agent for the remediation of petroleum-contaminated soil and selecting optimal conditions for the remediation of the high-efficiency degradation agent by utilizing an orthogonal remediation experiment. The bioremediation agent screened by the screening and remediation method can be applied to the bioremediation of the petroleum-contaminated soil with the salt content of 10 to 20 g / Kg. The method for the screening and remediation of the petroleum-contaminated soil bioremediation agent has high practical value, and can be widely applied in the fields of bioremediation of the petroleum-contaminated soil, beaches, petroleum production wastewater and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of petroleum-contaminated soil, and in particular relates to a screening method and a remediation method of bioremediation bacterial agents for petroleum-contaminated soil. Background technique [0002] Petroleum, as the "blood of industry", its industrial development and application are increasingly rapid. However, the pollution it brings, especially soil pollution, has also attracted much attention. At present, the most promising oil-contaminated soil treatment method recognized internationally is bioremediation. [0003] The research on the bioremediation technology of petroleum-contaminated soil began in the 1980s. This technology relies on a large number of microorganisms to use hydrocarbons in petroleum as carbon and energy sources to grow, and through their metabolism, the petroleum pollutants in the soil Degraded into carbon dioxide and water or converted into other harmles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/00B09C1/10
Inventor 杨玉楠韩冬
Owner BEIHANG UNIV
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