Method for screening and remediation of petroleum-contaminated soil bioremediation agent
A technology of oil pollution and soil biology, applied in the field of microbial remediation of oil-contaminated soil, can solve the problems that ordinary microorganisms cannot use high-salinity oil-contaminated soil for biological treatment, and achieve high repair effect
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[0052] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.
[0053] The screening and restoration method of a kind of bioremediation bacterium of petroleum-contaminated soil that the present invention proposes specifically comprises the following steps:
[0054] Step 1: Collection of sources of oil-degrading indigenous bacteria and halophilic bacteria;
[0055] The oil-contaminated soil at the discharge outlet of oilfield wastewater was used as the source of oil-degrading indigenous bacteria; the oil production wastewater and activated sludge of sewage treatment plant at the outlet of oilfield wastewater treatment plant were used as the source of halophilic bacteria.
[0056] Step 2: Screening of indigenous oil-degrading bacteria, such as figure 2 shown;
[0057] (1) Take 2g of the soil samples collected from 9 sampling points, add them to 9 Erlenmeyer flasks (containing glass beads) filled with 100mL steril...
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