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Method for cultivating gynogenetic carps

A technology of gynogenesis and cultivation method, applied in the field of fish cultivation, can solve problems such as heterosperm effect, achieve the effects of body back height, prolong the time of cold shock treatment, and improve the survival rate

Active Publication Date: 2011-04-13
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

The sperm used to activate the egg is generally heterologous sperm. Although it has been inactivated, there is a "heterosperm effect"

Method used

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  • Method for cultivating gynogenetic carps
  • Method for cultivating gynogenetic carps
  • Method for cultivating gynogenetic carps

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Embodiment Construction

[0014] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0015] A method for cultivating gynogenetic carp of the present invention, specifically comprising the following steps:

[0016] 1. Two to three months before the breeding season, select female carp and male bream individuals with obvious sexual maturity, good body shape, bright color, strong physique, and no disease or injury as broodstock for intensive breeding in special ponds. Feed feed to promote the good development of broodstock gonads.

[0017] 2. In the breeding season, select the above-mentioned parent fish with good signs and mature gonads to inject a mixture of chorionic gonadotropin (HCG) and lutein-releasing hormone analogue (LRHA) to artificially induce labor. Inject female carp first, when injecting female carp, the dosage of HCG is 800IU / kg-1000IU / kg, and the dosage of LRH-A is 20μg / kg-30μg / kg; inject male bream after 3h-4h, a...

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Abstract

The invention relates to cultivation gynogenetic fish, in particular to a method for cultivating gynogenetic carps. The method comprises the following steps of: performing artificial induced spawning on male megalobrama amblycephala and female ordinary carps which serve as male and female parent fish, mixing megalobrama amblycephala sperms inactivated by ultraviolet rays and mature ova of the ordinary carps, activating for 1 to 2 minutes, performing cold shock on the activated ova at the temperature of between 0 and 4 DEG C for 30 to 40 minutes, and incubating embryos obtained by the cold shock at the water temperature of between 20 and 21 DEG C to obtain diploid gynogenetic carps. The gynogenetic carps cultivated by the method have excellent descendant properties, high survival rate and stable inheritance, and have the great significance for the study on biological genetic breeding.

Description

technical field [0001] The invention relates to a method for cultivating fish, in particular to a method for cultivating gynogenetic fish. Background technique [0002] Artificial gynogenesis technology is used to quickly construct pure fish lines, which has very obvious effects; at the same time, it is also of great significance and value in the fields of fish sex determination and the establishment of gene maps. Generally, the breeding of gynogenetic fish is to use fish haploid eggs to be activated by sperm inactivated with genetic material, and then undergo chromosome doubling treatment to form diploid offspring that mainly rely on the genetic material of eggs to develop. The sperm used to activate eggs is generally heterologous sperm. Although it has been inactivated, there is a "heterosperm effect". This "heterospermy effect" may have a genetic improvement effect on gynogenetic offspring. In addition, the cold shock method is used for gynogenesis, so that the eggs go ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘少军刘筠邹拓谜肖俊肖军赵如榕罗凯坤陶敏张纯
Owner HUNAN NORMAL UNIVERSITY
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