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Method for comprehensively extracting tea saponin and tea polysaccharide from camellia oil seed cake or tea seed cake

A technology of comprehensive extraction of Camellia oleifera seed meal, which is applied in the fields of food, cosmetics and medicine, can solve the problems of poor color of tea saponin, ignoring the extraction of tea polysaccharides, and the quality of tea saponin can not meet the requirements of the market, so as to achieve easy volatilization, The effect of reducing the time to concentrate and recover ethanol

Inactive Publication Date: 2011-02-23
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tea saponin produced by most factories has poor color and low purity, which cannot meet the requirements of all walks of life, especially restricting the application of tea saponin in the field of medical technology
[0005] At present, many articles and patents have disclosed the method of extracting tea saponin by using camellia seed cake as raw material. Although these processes have made great progress, the quality of the obtained tea saponin product still cannot meet the requirements of the market.
In addition, in addition to being rich in tea saponin, camellia seed meal or tea seed meal also contains a large amount of tea polysaccharides. However, most people only study the extraction of tea saponin in camellia seed meal or tea seed meal, ignoring the content of tea polysaccharides. extract
Make camellia seed meal or tea seed meal can not be fully utilized, it is useless to really turn money into treasure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 500g of crushed Camellia oleifera seed cake, extract it twice with water at 70°C, the ratio of solid to liquid and heating time are 1:9 / 1.5h, 1:5 / 1.5h respectively, filter at 300 mesh, and centrifuge at 4500rad / min for 15min. Combine the water extracts, concentrate under reduced pressure at 60°C to a solid content of 15% as measured by an Abbe refractometer, slowly add 1 volume of industrial alcohol, stir well, place in a refrigerator at 4°C for alcohol precipitation, and filter the mixture through 500 mesh , to obtain the clear solution containing tea saponin and the tea polysaccharide precipitate respectively. After the tea polysaccharide precipitates and naturally volatilizes ethanol, it is freeze-dried to obtain the crude product of tea polysaccharide. The yield of polysaccharide is 9.09%, and the mass fraction is 20.67%. The solid content of the solution was 3%. After the solution was absorbed by the AB-8 macroporous resin, it was eluted with water, sodium hyd...

Embodiment 2

[0029] Take 500g of crushed Camellia oleifera seed cake, extract twice with ethanol at 80°C, the ratio of solid to liquid and heating time are 1:9 / 2.0h, 1:5 / 1.0h respectively, filter through 300 mesh, and extract with ethanol containing tea saponin Under the condition of reduced pressure, ethanol was reclaimed, and tea saponin was obtained by vacuum drying, with a yield of 18.29% and a purity of 68.79%. Concentrate under reduced pressure at ℃ to a solid content of 30%, slowly add 3 times the volume of industrial alcohol, stir well, ethanol precipitate the liquid, filter the mixture with a 1000-mesh filter cloth, and freeze-dry the precipitate after naturally volatilizing ethanol to obtain crude tea polysaccharides. The ratio is 4.58%, and the mass fraction is 18.49%.

Embodiment 3

[0031] Take 500g of crushed tea seed cake, extract twice with ethanol at 80°C, the ratio of solid to liquid and heating time are 1:9 / 2.0h, 1:5 / 1.0h respectively, filter through 300 mesh, and extract with ethanol containing tea saponin Liquid and ethanol extract flowed through the AB-8 macroporous adsorption resin, and the resin adsorbate was eluted with water, 0.2% mass concentration of sodium hydroxide, 20%, 40%, and 70% volume concentration of ethanol respectively, and collected different eluted The liquid was lyophilized, and the 70% ethanol eluate was lyophilized to obtain white crystal tea saponin with a purity of 89.6% and a yield of 4.95%. The slag obtained after filtration was subjected to two water extractions under the same conditions and then concentrated, combined the water extracts, concentrated at 60°C to a solid content of 30% under reduced pressure, slowly added 3 times the volume of industrial alcohol, and fully stirred , ethanol precipitation, filter the mixt...

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Abstract

The invention discloses a method for comprehensively extracting tea saponin and tea polysaccharide from a camellia oil seed cake or tea seed cake. In the method, a comprehensive extraction process for the tea saponin and the tea polysaccharide is researched by taking the degreased camellia oil seed cake or tea seed cake as raw materials. The method for extracting the tea saponin and tea polysaccharide comprises the following steps of: crushing the camellia oil seed cake or tea seed cake and performing water extraction and alcohol precipitation or alcohol extraction and water extraction to obtain rough tea saponin and tea polysaccharide; purifying the supernatant subjected to water extraction or alcohol extraction by using a macroporous adsorption resin chromatography column; eluting resin adsorbate with alkali and ethanol with gradually increasing volumetric concentration in turn; and collecting the ethanol eluent to obtain high-purity tea saponin. The method has a simple process and low production cost, and by the method, the high-purity tea saponin can be prepared and also another active product, namely the tea polysaccharide can be obtained.

Description

technical field [0001] The invention relates to a comprehensive extraction method for tea saponin and tea polysaccharide in camellia oleifera seed dregs or tea seed dregs. It belongs to the technical fields of food, cosmetics and medicine. Background technique [0002] Camellia oleifera is one of the four major woody oil plants in the world. It is a native tree species native to my country and has a cultivation history of more than 2,000 years. my country is the country with the largest cultivation area of ​​camellia oleifera in the world, the highest output and the most abundant varieties. According to statistics, there are about 50 million mu of camellia oleifera forests in my country, mainly distributed in Zhejiang, Anhui, Fujian, Jiangxi, He, Hubei, Hunan, Guangdong and Guangxi , Chongqing, Sichuan, Yunnan, Guizhou, Shaanxi and other low-mountain and hilly areas in 14 provinces (autonomous regions, municipalities) south of the Huaihe River and the Yangtze River, among wh...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H1/08C08B37/00C07H15/256
Inventor 魏新林王元凤俞兰郑月英陈辰
Owner SHANGHAI NORMAL UNIVERSITY
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