Chicken infectious laryngotracheitis bivalent nucleic acid vaccine and preparation method thereof
A nucleic acid vaccine, laryngotracheitis technology, applied in the directions of pharmaceutical formulations, genetic material components, introduction of foreign genetic material using carriers, etc. The effect of immune response strength, improving commission efficiency, and shortening incubation period
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[0049] A bivalent nucleic acid vaccine for chicken infectious laryngotracheitis, in which the complete genes of ILTV HN1 strain gB and ChIL-18 are respectively inserted into two multiple cloning sites of the eukaryotic expression vector pIRES, and the bivalent nucleic acid vaccine co-expresses chicken Infectious laryngotracheitis virus gB gene and chicken IL-18 gene.
[0050] The preparation method of chicken infectious laryngotracheitis bivalent nucleic acid vaccine, its steps are as follows:
[0051] (1) Design and synthesize a pair of primers for amplifying ChIL-18,
[0052] The upstream primer Y1 is: 5'-GTA GGATCC ATGAGCTGTGAAGAGAT-3',
[0053] The downstream primer Y2 is: 5'-TAC GTC GAC TTATAGGTTGTGCCTTTC-3',
[0054] BamHI and Sal I restriction sites (underlined part) were added to the upstream and downstream primers respectively, and the expected amplification length is 615bp;
[0055] Design and synthesize a pair of primers for amplifying ILTV gB,
[0056] The u...
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