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Method for performing tissue culture and rapid propagation on primula saxatilis

A technology for tissue culture and primula rock, applied in the field of plant tissue culture, can solve the problems of no relevant research and reports on tissue culture of primula rock, unable to meet production needs, low reproduction coefficient, etc., to shorten the seedling cycle, consistent Strong sex and the effect of improving reproduction coefficient

Active Publication Date: 2011-02-09
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Primrose rock is a perennial herbaceous flower, which is generally propagated by seeding and branching, but mainly by sowing. It is usually sown in early September, and enters the full flowering period in May of the following year after vegetative growth. The time from sowing to flowering is 8 months , long growth cycle and low reproduction coefficient
At the same time, these methods are far from meeting the production needs, and the seedlings are not neat
[0003] Tissue culture is an important way to rapidly propagate good plant varieties. There have been many reports on tissue culture of Primula genus, but there are no relevant researches and reports on tissue culture of Primula rocks at home and abroad. Therefore, it is necessary to study the Tissue culture of Primula and form a set of specialized rapid propagation technology system

Method used

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  • Method for performing tissue culture and rapid propagation on primula saxatilis
  • Method for performing tissue culture and rapid propagation on primula saxatilis
  • Method for performing tissue culture and rapid propagation on primula saxatilis

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Select the seeds of Primula rocks, disinfect them with 75% alcohol for 15 seconds under aseptic conditions, wash them with sterile water for 5-6 times, then disinfect them with 0.1% mercuric chloride solution by mass ratio for 6 minutes, and rinse them with sterile water for 5-6 times. Inoculate into 1 / 2MS, pH is 5.9, move to MS after one month, pH is 5.9, subculture, grow in MS for one month ( figure 1 ) to form Primula rock aseptic strong seedlings, the light conditions are natural scattered light 2500 ~ 3000Lux plus artificial auxiliary light 1500 ~ 2000Lux.

Embodiment 2

[0034] Select the seeds of Primula rocks, disinfect them with 75% alcohol for 30 seconds under sterile conditions, wash them with sterile water for 6 times, then disinfect them with 0.1% mercuric chloride solution for 8 minutes, rinse them with sterile water for 6 times, and inoculate them into MS medium, the pH is 5.9, and after one month, it was moved to MS for subculture. After one month of growth in MS, the sterile and strong seedlings of Primula rocks were formed. The light conditions were 2500-3000 Lux of natural scattered light and 1500-2000 Lux of artificial auxiliary light.

Embodiment 3

[0036] The axillary buds of Primula rock aseptic seedlings were used as explants for primary culture and subculture: select the axillary buds from the vigorously growing aseptic seedlings and inoculate them with MS+6-BA 2.5mg / l+NAA under sterile conditions On 1.0mg / l cluster bud induction medium, inoculate 3 buds per bottle; the light conditions are natural scattered light 3000Lux plus artificial auxiliary light source 1800±200Lux, light time 14h.

[0037] When the axillary buds were inoculated on the differentiation medium, new leaves were constantly drawn out, and the petiole of the new leaves was drawn out in 10 days, and the petiole was strong, and the base turned red, and after 2 weeks ( figure 2 ) began to differentiate clustered buds; 40 days after inoculation ( image 3 ), on the newly drawn petioles of the axillary buds, a large number of clustered buds are differentiated.

[0038] Statistical results show that after 2 months of inoculation on the medium of MS+6-BA ...

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Abstract

The invention provides a method for performing tissue culture and rapid propagation on primula saxatilis. The method comprises the following steps of: selecting wild primula saxatilis seeds collected from the Mountain Miyunwuling in Beijing as explants; sterilizing the seeds and then performing inoculation culture on the seeds, so that the seeds sprout to form sterile seedlings of the primula saxatilis; performing propagation on axillary buds on the sterile seedlings and then performing rapid propagation through cluster bud induction and subculture; or generating callus by inducing leaves or petioles, performing propagation on the callus and performing adventitious bud differentiation to perform rapid propagation. By the method for performing tissue culture and rapid propagation on the primula saxatilis provided by the invention, the seedling-cultivating production capacity of the primula saxatilis is greatly improved, seedling time and cost are reduced, and technical guarantee is provided for the storage of excellent individual plants of the primula saxatilis.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Primula saxatilis. Background technique [0002] Primula yansheng is a plant belonging to the Primula genus of Primulaceae, which is listed in the "Chinese Plant Red Book (Second Edition)" and "List of Key Protected Wild Plants in Hebei Province". It blooms in early spring, and the pink flowers have good ornamental value. It can survive summer and winter smoothly in the open field of Beijing. It is a very important ornamental flower and breeding material. Primrose rock is a perennial herbaceous flower, which is generally propagated by seeding and branching, but mainly by seeding. It is usually sown in early September, and enters the full flowering period in May of the following year after vegetative growth. The time from sowing to flowering is 8 months , long growth cycle and low reproduction coefficient. Simultaneously, t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张启翔赵妍潘会堂程堂仁王佳董玲玲
Owner BEIJING FORESTRY UNIVERSITY
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