Tissue-culture fast propagation method of musella lasiocarpa plant

Abstract

The invention provides a tissue-culture fast propagation method of a musella lasiocarpa plant, comprising the following steps of: inoculating inflorescences to be opened as explants into an induction medium to induce and culture calluses; then inoculating into an adventitious bud differential medium to be cultured and differentiated into buds; cutting the differentiated buds into single buds witha right amount of basal disc tissues, and inoculating into an enrichment medium to be cultured to obtain cluster buds; separately cutting the cluster buds into single plants, and inoculating into a rooting medium to be cultured into rooting seedlings; and acclimatizing the seedlings to obtain transplanting seedlings. The invention not only is easy and convenient for obtaining the materials, but also has large number of the explants because hundreds of the explants can be extracted from one inflorescence; the inflorescences tightly wrapped by bracts do not have germs because the germs are difficult to immerse into the inflorescences, therefore cleaning, disinfection and sterilization are very convenient; healthy transplanting seedlings are obtained through the sequential culture of the specifically-prepared induction medium, the adventitious bud differential medium, the enrichment medium and the rooting medium; and the survival rate reaches more than 90 percent after transplantation, therefore the demand quantity of the market on musella lasiocarpa can be completely satisfied.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of plants, in particular to a method for tissue culture and rapid propagation of T. chinensis plants, and belongs to the technical field of plant tissue culture. Background technique [0002] Musella lasiocarpa, also known as Musella lasiocarpa, Musella lasiocarpa, is a large perennial herbaceous plant with rhizomes in the Musaceae genus Musella lasiocarpa. It is a unique single genus species in China, mainly distributed In the mountainous slopes of central and western Yunnan at an altitude of 1500-2500 meters, there are also a small amount of distribution in parts of Sichuan Province near Yunnan. Its flower shape is peculiar, the flower color is bright, and the ornamental period is as long as more than 200 days. It is a rare flower with great ornamental value and local characteristics. It also has various functions such as medicine, food, feed, textile, and water and soil conse...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is: because the number of sucking buds on the T. chinensis plant is relatively limited, generally only 3 to 6 sucking buds will sprout from each T. chinensis plant, and the bases of the sucking buds are usually buried deep in the soil. Not only is it difficult to obtain materials, but it is often difficult to clean and sterilize because of carrying a large number of germs, and even impossible to perform tissue culture, so it is difficult to obtain sterile materials, which greatly affects the yield of T. The demand has hindered the promotion and application of this golden lotus with high ornamental value, great development potential and wide application.