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Granular type compound microbial bacterial agent as well as preparation method and application thereof

A composite microbial inoculum and granular technology, applied in bacteria, organic fertilizers, etc., can solve the problems of low number of viable bacteria and large differences in water content after adsorption, and reduce and absorb residual pesticides and toxic substances. The effect of increasing fertilizer intensity and improving utilization

Inactive Publication Date: 2014-03-05
SHANGHAI NUOTONG INFORMATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, scientific research institutes and enterprises engaged in the production of biological bacterial agents basically use peat, lignite, and weathered coal as adsorption carriers, but the water content in these substances is very high, even if they are dried at high temperature, the water content is still 15-20% At the same time, due to their different origins, varieties and mining seasons, the water content varies greatly, so the proportion of ingredients and the hydrotrope used in the production process will also change accordingly, and the number of viable bacteria in the bacterial agent after adsorption Low

Method used

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  • Granular type compound microbial bacterial agent as well as preparation method and application thereof
  • Granular type compound microbial bacterial agent as well as preparation method and application thereof
  • Granular type compound microbial bacterial agent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Strain fermentation

[0052] Bacillus subtilis (Bacillus subtilis), Bacillus megaterium (Bacillus megaterium), jelly-like Bacillus (Bacillus mucilaginosus), three strains were respectively subjected to slant culture, shaker expansion culture, seed tank fermentation and fermenter fermentation, After fermentation, the number of viable bacteria in the bacterial liquid reaches 3.5-4.5 billion / ml; (for strain fermentation, please refer to figure 1 )

[0053] 2. Bacterial liquid adsorption: the fermented bacterial liquid of a single strain is adsorbed with medical stone respectively in the adsorption stirring equipment, (the adsorption ratio is by Bacillus subtilis bacterial liquid: medical stone=1:5 (L / kg) , the adsorption ratio is by bacillus megaterium bacterium liquid: medical stone=1: 5 (L / kg), and the adsorption ratio is by jelly-like bacillus: medical stone=1: 5 (L / kg)), then stir Evenly, then mix and mix according to the ratio of 2:2:1; (for the preparation of po...

Embodiment 2

[0070] In the preparation of powdery composite microbial bacterial agent: the fermented bacterium liquid of single strain is adsorbed with medical stone respectively in the adsorption stirring equipment, (adsorption ratio is by Bacillus subtilis bacterium liquid: medical stone=1: 4( L / kg), the adsorption ratio is according to bacillus megaterium bacteria liquid: medical stone=1: 6 (L / kg), and the adsorption ratio is according to jelly-like bacillus: medical stone=1: 5 (L / kg) ), then stir evenly, then mix and mix according to the ratio of 2:3:1. In the production ratio of the composite microbial bacterial agent, the ratio between them is powdery composite microbial bacterial agent: phosphate rock powder: medium and trace elements=80:14:6. Others are with embodiment 1.

Embodiment 3

[0072] In the preparation of powdery composite microbial bacterial agent: the fermented bacterium liquid of single bacterial species is adsorbed with medical stone respectively in the adsorption stirring equipment, (adsorption ratio is by Bacillus subtilis bacterium liquid: medical stone=1: 6( L / kg), the adsorption ratio is according to bacillus megaterium bacteria liquid: medical stone=1: 6 (L / kg), and the adsorption ratio is according to jelly-like bacillus: medical stone=1: 5 (L / kg) ), then stir evenly, then mix and mix according to the ratio of 1:1:1. In the production ratio of the composite microbial bacterial agent, the ratio between them is powdery composite microbial bacterial agent: phosphate rock powder: medium and trace elements=90:8:2. Others are with embodiment 1.

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Abstract

The invention relates to a granular type compound microbial bacterial agent which comprises the following components in percentage by weight: 80-90 percent of powdered compound microorganism bacterial agent, 8-14 percent of phosphate rock powder and 2-6 percent of trace element, wherein the powdered compound microorganism bacterial agent is prepared by adsorbing zymocyte fluid with a single strain respectively with a medical stone, and the strains are bacillus subtilis, bacillus megateria and bacillus mucilaginosus. The invention also provides a preparation method and application of the granular type compound microbial bacterial agent. The invention provides a good carrier for the production of microbial bacterial agent granules; the medical stones have various characteristics of good adsorbability, leachability, mineralization, bioactivity, and the like and provide good carriers for the adsorption of the microbial bacterial agent; a produced fertilizer has better appearance, and the intensity of the fertilizer is obviously enhanced; and the invention greatly improves the utilization rate and the long-term effectiveness of the fertilizer and is beneficial to storing and transporting products.

Description

【Technical field】 [0001] The invention relates to a granular composite microbial bacterial agent and a production method thereof, in particular to a composite microbial bacterial agent using medical stone as an adsorption carrier for microbial fermentation liquid, a production method and application thereof. 【Background technique】 [0002] Medical stone is a loose layered, veined, and lenticular structure formed by the original rock through alteration and weathering. It contains a variety of mineral elements that are beneficial to plant growth and development. It has a large specific surface area and has a porous and Sponge-like structure, at the same time, in these structures, tetrahedrons and octahedrons are arranged alternately, and Al in the lattice 3+ instead of Si 4+ As a result, excess negative charges appear, and additional cations are needed to balance the negative charges. These cations are loosely combined with the crystal lattice, so medical stone has good adsor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08
Inventor 闫龙翔
Owner SHANGHAI NUOTONG INFORMATION TECH CO LTD
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