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I-type canine adenovirus attenuated vaccine strain and application thereof

A technology for canine adenovirus and attenuated vaccines, which can be applied to antiviral agents, inactivation/attenuation, nervous system diseases, etc. It can solve the problems of high cost and low antigen specificity, and achieve good protection and immune protection effect, good immunogenicity

Inactive Publication Date: 2012-01-11
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the above vaccines have disadvantages such as high cost and poor antigenic pertinence, so it is imminent to develop new and efficient CD vaccines

Method used

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  • I-type canine adenovirus attenuated vaccine strain and application thereof
  • I-type canine adenovirus attenuated vaccine strain and application thereof
  • I-type canine adenovirus attenuated vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1I

[0014] Isolation and identification of embodiment 1 type I canine adenovirus (CAV-H) isolate

[0015] 1 Materials and methods

[0016] 1.1 Disease material: The affected dog came from Heilongjiang Province. The clinical manifestations were elevated body temperature, watery eyes and nose, and bloody stool. The sick dog died within 3 days of the illness. Intestinal contents were collected for virus isolation.

[0017] 1.2 Cells: MDCK (generation 74-94, the same below) cell line was purchased from China Veterinary Drug Control Institute.

[0018] 1.3 Experimental animals: 2-4 month-old puppies (CAV antibody titer ≤ 1:2) were selected and provided by the Department of Experimental Zoology of Harbin Medical University. After deworming and 1w observation, it was determined that the healthy ones were available.

[0019] 1.4 Serum: canine anti-CAV (SN≥1:16) standard positive serum and negative serum were made by the inventor's laboratory.

[0020] 1.5 Isolation of virus: Take the i...

Embodiment 2I

[0079] The cultivation of embodiment 2 type I canine adenovirus attenuated vaccine strain CAV-HR

[0080] 1 Materials and methods

[0081] 1.1 Test materials The test animals are healthy dogs aged 2 to 4 months (CAV antibody titer ≤ 1:2); anti-canine adenovirus-specific serum and 1% red blood cell suspension are prepared by our laboratory, and the strong toxicity is CAV-H 6 generation.

[0082] 1.2 Cultivation and identification of CAV-H attenuated strain

[0083] 1.2.1 Cultivation of attenuated CAV-H strain

[0084] CAV-H was passaged through MDCK cells to passage 110, during which clonal purification was carried out 3 times.

[0085] 1.2.2 Morphological observation of attenuated CAV-H strain

[0086] Centrifuge the harvested CAV-H 5, 10, 20, 30, 50, 70, 90, and 110 passage virus cell cultures at 5000r / min for 30min, take the supernatant and ultracentrifuge at 20000r / min, centrifuge at 4°C for 2h, and use for precipitation Appropriate amount of PBS (pH 7.0) suspended, ph...

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Abstract

The invention discloses an I-type canine adenovirus attenuated vaccine strain and application thereof. In the invention, the separated I-type canine adenovirus attenuated vaccine strain is carried out passage and cloned on MDCK (Madin-Darby Canine Kidney), and attenuated strains (CAV-HR) are cultured. The security and immunogenicity test result shows that the attenuated strains CAV-HR can providegood protection force for dogs attacked by isogeny virulent virus. The attenuated strains (CAV-HR) has favorable immunogenicity, can be prepared into a single seed or combined seeds, and can effectively prevent or treat the diseases caused by the I-type canine adenovirus. The attenuated vaccine strain has the advantages of stable hereditary, lasting immunity, good effect, security, reliability, long preservation period and the like.

Description

technical field [0001] The present invention relates to a strain of virus attenuated vaccine strain, especially relate to an attenuated vaccine strain CAV-HR weakened by type I canine adenovirus virulent strain CAV-H, the present invention also relates to this attenuated vaccine strain in the prevention or treatment of The application in various diseases caused by type I canine adenovirus belongs to the field of prevention or treatment of type I canine adenovirus. Background technique [0002] Canine adenovirus type I was first discovered in 1925. In 1984, the virus was isolated for the first time in my country, which confirmed the infection of canine adenovirus type I in dogs in my country (Yin Zhen, Liu Jinghua. Animal Virology (Second Edition). Beijing: Science Press, 1997, 757-762 .). Type I canine adenovirus can clinically cause canine infectious hepatitis (Webe J.DNA vaccine.Business Week, 1996, 2:6.), fox and bear encephalitis (Xia Chengzhu, Fan Quanshui, Hu Rongliang...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/08A61K39/235A61P31/20A61P1/16A61P25/00C12R1/93
Inventor 刘大飞张洪英曲连东王牟平刘立奎
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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