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Active lactobacillus casei beverage and preparation method thereof

A technology of Lactobacillus casei and beverages, which is applied in the field of active Lactobacillus casei beverages and its preparation, can solve the problems of long fermentation period, slow fermentation acid production, discount of prebiotic function, etc., and achieve reduced risk of fermentation failure, rapid pH value drop, Effect of production cost reduction

Active Publication Date: 2012-08-22
武汉光明乳品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are two main problems in the production of probiotic beverages in the market: ordinary lactic acid bacteria beverages cannot meet the quantity and activity of lactic acid bacteria that reach and colonize the intestinal tract, so their probiotic functions are greatly reduced, and the product price and gross profit rate are also low. 100mL The market price is about 0.5 to 1.0 yuan; the Lactobacillus casei probiotic beverage produced by conventional fermentation technology can satisfy the quantity and activity of lactic acid bacteria colonized in the intestinal tract, and the probiotic function is remarkable. The product price and gross profit rate are relatively high. At 2.0 yuan, but the fermentation cycle is too long (about 72h), the acid production in the early stage of fermentation is slow, and the requirements for fermentation equipment are relatively strict.
[0007] In "A Preparation Method for Active Lactic Acid Bacteria Beverage" (publication number: CN 1899053A), Lactobacillus casei subsp. Actual production: Streptococcus thermophilus, Lactobacillus bulgaricus, Lactobacillus acidophilus and Lactobacillus reuteri are used for mixed fermentation at 42-45°C. Gastric acid resistance, bile acid resistance and salt resistance are weaker than Lactobacillus casei, and the probiotic function of the final product will also be weakened

Method used

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  • Active lactobacillus casei beverage and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] (1) Skimmed milk powder was reduced and hydrated at 12.0%, 400g / bottle was divided into 500mL Erlenmeyer flasks, sterilized in a water bath at 96°C for 120min, cooled to 37°C for later use.

[0077] (2) Under aseptic conditions, according to the total inoculation amount of 0.04‰ and the ratio shown in Table 1, insert different strains into each Erlenmeyer flask, mix evenly, and ferment at 37°C for 36 hours, then measure the acidity and cheese milk of the fermentation system The number of live bacilli was shown in Table 1.

[0078] Table 1 The combination of different strains and their effects on fermentation

[0079]

[0080] 9

[0081] The results showed that when Lactobacillus casei L.casei-01 was matched with Streptococcus thermophilus BODY-3 and TA40 at a ratio of 1:1 to 100:1, it had a good synergistic fermentation effect, and the acidity increased rapidly, and L. The number of live bacteria in casei reached the highest. When Lactobacillus casei an...

Embodiment 2

[0083] (1) Skim milk powder is reduced and hydrated at 12.0%, and 400g / bottle is divided into 500mL conical flasks, and carbon sources and metal ions of different types and concentrations are added to different conical flasks as shown in Table 2, and stirred evenly and fully Dissolve and sterilize in a water bath at 96°C for 120 minutes, then cool to 37°C for later use.

[0084] Table 2 Addition amount of different carbon sources and inorganic salts

[0085] Numbering

[0086] (2) According to the total amount of inoculation of 0.04‰, the ratio of L.casei-01:S.t BODY-3=10:1, insert the starter, ferment for 36 hours at 37°C, measure the acidity and the content of Lactobacillus casei after the fermentation ends, The results are shown in Table 3.

[0087] Table 3 Effects of different carbon sources and inorganic salt additions on the fermentation of L.casei

[0088] Numbering

[0089] Adding carbon sources and metal ions to the fermentation system can both...

Embodiment 3

[0091] (1) Skim milk powder is reduced and hydrated at 12.0%, 400g / bottle is divided into 500mL triangular flasks, and active polypeptides of different types and concentrations are added to different triangular flasks as shown in Table 4, stirred evenly, and sterilized in a 96°C water bath After 120 minutes, cool down to 37°C for later use.

[0092] (2) According to the total inoculum size of 0.04‰, the ratio of BD-II: TA40=50: 1 was inserted into the starter, and after 36 hours of fermentation at 37°C, the acidity of the fermentation system and the content of Lactobacillus casei were measured. The results are shown in Table 4.

[0093] Table 4 Effect of adding different active polypeptides on fermentation

[0094] Numbering

[0095] Active polypeptides can significantly promote the proliferation of Lactobacillus casei, and soybean polypeptides are slightly superior. About 2‰ soybean polypeptides or fish collagen peptides can promote the increase of fermented lactic...

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Abstract

The invention discloses an active lactobacillus casei beverage and a preparation method thereof and relates to an active probiotics beverage in the technical field of microorganism fermentation. The beverage comprises the following components in percentage by mass: 25 to 40 percent of fermented milk, 8 to 12 percent of white granulated sugar, 0.3 to 0.5 percent of stabilizing agent, 0.03 to 0.05 percent of food flavor and 50 to 70 percent of purified water. The preparation method comprises the following steps of: 1, preparing base materials; 2, fermenting the base materials; (3) preparing anddisinfecting sugar liquor; and (4) preparing the beverage. Compared with the prior art, the method has the advantages of remarkably shortened product fermentation time, remarkably increased bacteria content of lactobacillus casei, lowered product cost and fermentation pollution risk, lowered equipment investment and suitability for producing the lactobacillus casei probiotics beverage under the conventional equipment condition by national dairy enterprises.

Description

technical field [0001] The invention relates to an active probiotic drink in the technical field of microbial fermentation, in particular to an active Lactobacillus casei drink and a preparation method thereof. Background technique [0002] Active lactic acid bacteria drink is a fermented beverage containing active probiotics, which can not only alleviate lactose intolerance, but also contain fully degraded small molecular proteins and polypeptides, which can promote the digestion and absorption of milk protein. At the same time, the gut-regulating function of probiotics has made this type of product gradually recognized and welcomed by consumers. [0003] It cannot be ignored that in active probiotic drinks, the type and quantity of probiotics will obviously affect the probiotic function and health care effect of the drink. Most lactic acid bacteria, such as Streptococcus thermophilus and Lactobacillus bulgaricus used in ordinary lactic acid bacteria drinks, yogurt, etc., ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23C9/13
Inventor 王荫榆马成杰杜昭平龚广予
Owner 武汉光明乳品有限公司
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