Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof
A technology of composite bacterial agents and microbial strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of waste non-point source pollution, increase production, etc.
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Embodiment 1
[0033] Example 1: Rhodopseudomonas palustris (Rhodop seudanonas palustris) with the number 1.2352 and Candida utilis with the number 2.1180 disclosed in the strain catalog were purchased from China General Microorganism Culture Collection Center (CGMCC) (Candida utilis), Lactobacillus acidophilus (Lactobacillus acidophilus) number 1.1854, Bacillus subtilis (Bacillus subtilis) number 1.1630 and Streptomyces microflavus var. lactosus);
[0034] Step 1, the purification culture of various bacteria, the medium used, the culture temperature and the time of culture are respectively as follows:
[0035] (1) The culture medium for Rhodopseudomonas palustris is 0259ATYP agar medium, medium formula: KH 2 PO 4 1.0g, CaCl 2 0.1g, NaHCO 3 3.0g, sodium acetate 1.0g, NH 4 Cl 0.5g, NaCl 1.0g, trace element solution 1.0ml and vitamin solution 1.0ml;
[0036] Among them, trace element solution: FeCl 2 4H 2 O 1.8g, CoCl 2 ·6H 2 O 0.25g, NiCl 2 ·6H 2 O CuCl 2 2H 2 O 0.01g, MnCl ...
Embodiment 2
[0053] Example 2, using the composite microbial agent obtained in Example 1 to prepare bio-organic fertilizer, prepare 200 kg of lignite, 500 kg of poultry manure, 300 kg of straw powder, and 3 kg of composite bacterial agent.
[0054] Follow the steps below to produce:
[0055] (1) Mix 200kg of lignite, 500kg of poultry manure, and 300kg of straw powder evenly, add 3kg of compound bacterial fertilizer during the stirring process, and ferment in the fermentation workshop for 48 hours;
[0056] (2) When the temperature rises to 72°C, use the automatic compost turning machine to start composting, and in the next 8 days, turn the compost every 12 hours;
[0057] (3) During the turning process, the high temperature generated by microbial fermentation evaporates the water, and aerobic fermentation is carried out at the same time. When the water content in the compost drops to 20%, the product inspection is carried out;
[0058] (4) Quantitative packaging is done for products that ...
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