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Rapid separation method of antagonistic bacteria against pathogens of konjac soft rot disease

A technology of soft rot and pathogenic bacteria, applied in the field of rapid separation of antagonistic bacteria of konjac soft rot pathogens, achieving low cost and simple operation

Inactive Publication Date: 2010-11-24
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

There are many types of microorganisms in nature, how to quickly and effectively obtain the antagonistic bacteria of the pathogenic bacteria of konjac soft rot has not been reported yet

Method used

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Experimental program
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Effect test

Embodiment 1

[0017] A method for rapidly isolating antagonistic bacteria of konjac soft rot pathogenic bacteria, the steps are:

[0018] A. Standard sample selection: select konjac tissue with typical soft rot symptoms, and cut 0.5-1.5cm tissue block at the junction of disease and health at the frontier of disease expansion;

[0019] B. Tissue disinfection: Rinse the tissue block with tap water, use 75% (volume ratio) alcohol to sterilize for 60 or 70 or 80 or 90 seconds, then rinse with sterile water for 2 or 3 or 4 times, and put it on a tray with absorbent paper Use a scalpel to cut into tissue pieces of about 0.5×0.5cm on the sterile operating table, and connect them to the nutrient agar medium;

[0020] C. Acquisition of bacteria: Separate the bacteria growing around the tissue block using the plate streak separation method, and observe after culturing in a 24-28°C incubator for 48 or 51 or 54 or 57 or 60 or 64 or 67 or 70 or 72 hours According to the bacterial culture characteristic...

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PUM

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Abstract

The invention discloses a rapid separation method of antagonistic bacteria against pathogens of konjac soft rot disease, which comprises the following steps: A) taking konjac tissues infected by the soft rot disease; B) inoculating the tissues at the interface of diseased and healthy tissues onto a nutrient agar culture medium after carrying out surface disinfection; C) streaking the bacteria grown out on the sides of the tissues, and selecting a single colony for purification and preservation; D) adopting a distinctive Erwinia crystal violet polypectin selective culture medium (CVP) to screen the pathogens of the konjac soft rot disease; E) adopting the point connection method to carry out primary screening of antagonistic bacteria; and F) adopting the oxford cup method to carry out secondary screening of the antagonistic bacteria. The method has simple operation and easy operation, and can rapidly and effectively screen out the antagonistic bacteria against the pathogens of the konjac soft rot disease and simultaneously provide a concept for rapid screening of the antagonistic bacteria against other crop diseases.

Description

technical field [0001] The invention relates to a method for rapidly separating antagonistic bacteria of konjac soft rot pathogenic bacteria, and belongs to the technical field of plant disease prevention and control. The pathogenic bacteria of konjac soft rot disease described in the present invention is caused by the infection of Pectobacterium Species. Background technique [0002] Konjac is a monocotyledonous perennial herb mainly distributed in Southeast Asia and Africa. The rich glucosidan in konjac bulbs makes it widely used in food, medicine, chemical industry and agriculture. With the increasing demand for raw materials in the konjac processing industry, the planting area continues to expand, and the occurrence of soft rot is becoming more and more serious, which has become a major obstacle to the development of the konjac industry. [0003] At present, the control of soft rot in agricultural production mainly includes agricultural control and chemical control. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 吴金平顾玉成向发云曾祥国宋志红
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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