Test kit for identifying single nucleotide polymorphism of target nucleic acid

Abstract

The invention discloses a test kit for identifying single nucleotide polymorphism of target genes including a CYP2C9 gene and a VKORC1 gene in a biological sample. The kit comprises a first group of probes and a second group of probes, wherein a first probe in the first group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:3, 4 and 5 and a complementary sequence thereof is at least 80 percent; a second probe in the first group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:6, 7 and 8 and a complementary sequence thereof is at least 80 percent; a first probe in the second group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:11, 12 and 13 and a complementary sequence thereof is at least 80 percent; and a second probe in the second group of probes has a nucleotide sequence of which the similarity with any one of nucleotide sequences SEQ ID NO:14, 15 and 16 and a complementary sequence thereof is at least 80 percent. The reagent kit can also comprise a first primer pair and a second primer pair.

Description

technical field [0001] The present invention is generally related to determining the appropriate starting dose or suitable dose for each individual based on the simultaneous two genotypes of the individual. Specifically, the present invention is through a test kit, especially a test kit for identifying single nucleotide polymorphisms of target genes including CYP2C9 gene and VKORC1 gene in a biological sample, To identify the single nucleotide polymorphisms of at least two target nucleic acids in this individual, and then determine the appropriate starting dose or applicable dose for this individual. Background technique [0002] A group of mutated single nucleotides at the positions of the gene sequence, also known as "single nucleotide polymorphisms (SNPs)", are distributed throughout the gene sequence. A single nucleotide polymorphism can also be allelic, that is, due to the existence of a genetic polymorphism, some individuals in a species will have a non-variant sequen...

AI Technical Summary

Problems solved by technology

However, due to the low frequency of these two CYP2C9 variants in Asians, polymorphisms in this gene cannot fully explain the differences in warfarin dosage requirements, especially the lower warfarin requirements in Asians ( See Yuan et al., Human Moleculargenetics, 14(13):1745-1751, 2005 and Nasu et al., Pharmacogenetics, 7(5):405-409, 1997)

Images