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Active extract containing trilobatin and application thereof

A technology of extract and trilobatin, applied in the field of active extract containing trilobatin and its application, can solve the problems of low cost, simplicity and no process

Active Publication Date: 2015-07-15
CHANGZHOU HI TECH DISTRICT MULTIPLE DIMENSION IND TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there is still no extraction method for the preparation of high-content trilobatin with simple process, low cost and suitable for large-scale production, and there is no high-content active extract of trilobatin for the preparation of applications in the treatment of diabetes

Method used

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  • Active extract containing trilobatin and application thereof
  • Active extract containing trilobatin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 macroporous resin refining trilobatin

[0052] Add 8 times the amount of 70% ethanol aqueous solution to the tender leaves of Cloveria fringa, soak for 2 hours, heat to reflux state, and reflux for 1 hour, filter; add 6 times the amount of 70% ethanol aqueous solution to the filter residue and heat to reflux state And reflux for 1 hour, filter; repeat this process 1 time. The filtrates were combined, concentrated under reduced pressure to recover ethanol, and trilobatin extract was obtained.

[0053] Put 30g of trilobatin dry extract in 100ml of distilled water, heat to 80°C and keep stirring to completely dissolve the trilobatin, then filter while hot to obtain a brownish-brown clear solution, let it stand and cool to 20-40°C, and dissolve the solution Add it to the head of medium-polarity macroporous adsorption resin (DM-301), elute with distilled water to remove some water-soluble impurities and pigments, then elute with 20%-50% ethanol aqueous solution...

Embodiment 2

[0059] Example 2 Polyamide Refined Trilobatin

[0060] Add 10 times the amount of 80% ethanol aqueous solution to the young leaves of Cloveria fringa, soak for 30 minutes, heat to reflux state, and reflux for 1 hour, filter; add 5 times the amount of 80% ethanol aqueous solution to the filter residue, heat to reflux state and Reflux for 1 hour and filter; this process is repeated once. Combine the filtrates, recover ethanol under normal pressure, and concentrate to obtain trilobatin extract.

[0061] Put 30g of trilobatin dry extract in 100ml of distilled water, heat to 80°C and keep stirring to completely dissolve the trilobatin, then filter while hot to obtain a brownish-brown clear solution, let it stand and cool to 20-40°C, and dissolve the solution Add to the head of polyamide, elute with distilled water to remove some water-soluble impurities and pigments, then elute with 20%-40% ethanol aqueous solution, TLC detection, collect the eluate at the end, concentrate and fil...

Embodiment 3

[0063] Make tablet of the present invention by following formula: (all content used is weight percent composition)

[0064] Active extract with 70% trilobatin 54.5%

[0065] Microcrystalline Cellulose 42.0%

[0066] PVP-k30 1.0%

[0067] Magnesium Stearate 0.5%

[0068] Sodium Carboxymethyl Starch 2.0%

[0069] Mix trelobatin extract, microcrystalline cellulose, and part of carboxymethyl starch sodium, add PVP-k30 ethanol solution to make soft material, granulate through a 20-mesh sieve, granulate after air-drying at 60°C, add carboxymethyl starch Sodium methyl starch and magnesium stearate are mixed evenly and pressed into tablets.

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Abstract

The invention discloses an active extract which is extracted from fagaceae and other plants by using 5 to 95% alcohol aqueous solution serving as a solvent, wherein the content of the trilobatin in the active extract is between 30 and 99.9%. The invention also discloses a preparation method for the active extract and application thereof in preparing a medicament for treating diabetes mellitus, diabetes nephropathy and hepatic edema.

Description

technical field [0001] The present invention relates to a high-content active extract mainly containing trilobatin extracted from plants such as Fagaceae and a preparation method thereof, and an active extract used in the preparation of pharmaceutical compositions for treating diabetes, diabetic nephropathy and hepatic edema. application. Background technique [0002] Diabetic nephropathy (diabetic nephropathy, DN), diabetic glomerulosclerosis, is a common and refractory microvascular complication of diabetes, and is the main cause of death in diabetes. In the west, DN has become the primary cause of end-stage renal disease; about 1 / 3 of type I diabetes and 1 / 5 of type II diabetes in the United States will eventually develop diabetic nephropathy (Song Chunguang, Chinese Medicine Research and Information, 2005, 7 (10):19-20, 26). [0003] The evolution of renal function and structural lesions in diabetic patients can be divided into the following five stages: Stage I: chara...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/45A61K36/73A61K36/87A61K36/185A61P1/00A61P3/00A61P3/10A61P7/06A61P7/10A61P9/04A61P9/06A61P9/10A61P9/12A61P11/00A61P13/12A61P17/06A61P17/10A61P35/00A61P29/00A61P19/02A61P19/06A61P37/02A61P37/08
CPCY02A50/30
Inventor 顾书华孙伟新赵维民蒋华良
Owner CHANGZHOU HI TECH DISTRICT MULTIPLE DIMENSION IND TECH INST
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