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Triiodothyronine detection reagent kit and use method thereof

A detection kit, the technology of diiodothyronine, which is applied in the field of triiodothyronine detection kits, can solve the problems of expensive instruments and complicated automatic design

Active Publication Date: 2010-10-20
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the separation and cleaning involved in the reaction process, the automation design is complicated and the equipment is quite expensive
In addition, the stability of the luminescent matrix is ​​also a big problem

Method used

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  • Triiodothyronine detection reagent kit and use method thereof
  • Triiodothyronine detection reagent kit and use method thereof
  • Triiodothyronine detection reagent kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1 Preparation of T2-coated luminescent particles

[0109] 1) Suspension treatment of luminescent particles: absorb a certain amount of carboxyl luminescent particles and centrifuge in a high-speed refrigerated centrifuge, discard the supernatant, add a certain amount of MES buffer, ultrasonically break until the particles are resuspended, add MES buffer to adjust the luminescent particles Concentration to 100mg / ml.

[0110] 2) Antibody treatment: T2 was dialyzed against 0.05M MES buffer at pH 6.0 (hereinafter referred to as MES buffer). After the dialysis was completed, the concentration was measured and adjusted to 2 mg / ml.

[0111] 3) Mix MES buffer, 100mg / ml luminescent particle suspension (MES buffer) and 2mg / ml T2 (MES buffer) at a volume ratio of 1:10:1, and mix quickly to obtain a reaction liquid.

[0112] 4) Prepare 40mg / ml EDAC solution with MES buffer, add according to the ratio of 100mg / 100uL EDAC to the luminescent particles, mix quickly, and rotat...

Embodiment 2

[0166] Example 2 Preparation of biotin-labeled antibody

[0167] Preparation:

[0168] 1) Antibody treatment: pipette a certain amount of anti-T3 (labeled) into a dialysis bag with a molecular weight cut-off of 14000D. Place 100 times the volume of 0.1M NaHCO3 solution in a chromatographic freezer at 2-8°C for dialysis, replace the 0.1M NaHCO3 solution twice, and dialyze for 4-5 hours each time; suck out the dialyzed anti-T3 (marker) and transfer it to a clean In a centrifuge tube, centrifuge at 10000g for 10min at 2-8°C; transfer the supernatant to another clean centrifuge tube, take a sample to measure the antibody concentration, and adjust the concentration to 1mg / ml.

[0169] 2) Labeling: Take the treated 1 mg / ml anti-T3 (labeled) and the prepared 16.17 mg / ml Biotin (DMSO) solution, mix them according to the volume ratio of 10000:54, and mix quickly. Stand at 2-8°C for 12-16 hours.

[0170] 3) Dialysis: put the reacted biotin-labeled antibody into a dialysis bag with a ...

Embodiment 3

[0177] Example 3 Preparation of Photosensitive Microparticles Coated with Avidin

[0178] Photosensitive particles: use photosensitive particles with a particle size of 220±40nm (PentaTek, USA)

[0179] Preparation:

[0180] a. Treatment of photosensitive particle suspension: absorb a certain amount of photosensitive particles and centrifuge in a high-speed refrigerated centrifuge, discard the supernatant, add a certain amount of MES buffer, ultrasonically sonicate on the ultrasonic cell disruptor until the particles are resuspended, and then add MES buffer Adjust the photosensitive particle concentration to 100mg / ml.

[0181] b. Preparation of avidin solution: weigh a certain amount of Avidin, add MES buffer to dissolve to 8mg / ml.

[0182] c. Mixing: Mix the treated photosensitive microparticle suspension, 8 mg / ml Avidin and MES buffer at a volume ratio of 2:5:1, and mix quickly to obtain a reaction solution.

[0183] d. Reaction: Prepare 25mg / ml NaBH in MES buffer 3 CN s...

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Abstract

The invention relates to an auxiliary diagnosis reagent kit of thyroid diseases, which discloses a triiodothyronine detection reagent kit. The triiodothyronine detection reagent kit comprises triiodothyronine detection particles, biotin marked anti-triiodothyronine antibodies and dissociation agents, wherein the triiodothyronine detection particles are diiodothyronine covered luminous particles, and the dissociation agents are 8-anilino group-1-ammonium naphthalenesulfenesulfonate. The invention also discloses a use method of the triiodothyronine detection reagent kit. The reagent kit of the invention can be combined with other blood serum and clinic information to be used for auxiliary diagnosis on the thyroid diseases and the monitoring on the relevant patient treatment effect.

Description

technical field [0001] The invention relates to a detection kit for triiodothyronine, in particular to a detection kit for triiodothyronine based on the principle of light-excited chemiluminescence and a use method thereof. Background technique [0002] Thyroid disease is a group of more common endocrine diseases, including iodine deficiency goiter, goiter caused by other reasons, hyperthyroidism, thyroid tumor, thyroiditis, hypothyroidism and so on. The incidence of thyroid disease is relatively high. [0003] Hyperthyroidism, also known as hyperthyroidism, is a very common clinical endocrine disease. It refers to the enhancement of thyroid function caused by various reasons, excessive secretion of thyroid hormones or increased levels of thyronine (T3, T4) in the blood caused by the body's nervous system, circulatory system, digestive system, cardiovascular system, etc. Systemic series of hypermetabolic syndrome and symptoms of hyperexcitability and ocular symptoms. [0...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/542
Inventor 张伟王海蛟赵卫国
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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