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Method for preparing swine multi-valent antiserum compound preparation

A compound preparation and antiserum technology, applied in antiviral agents, antibodies, pharmaceutical formulations, etc., can solve problems such as poor inactivation effect, high technical equipment requirements, and high cost of separation technology

Active Publication Date: 2012-06-20
SHANXI ZHAOYI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to improve the preventive effect of pig multiple antiserum products, and in order to overcome the disadvantages of high cost of exogenous microorganism isolation technology, high technical equipment requirements and poor inactivation effect of viruses, bacteria and mycoplasma in existing pig multiple antiserum products, the present invention A method for preparing a porcine multiplex antiserum compound preparation is provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of 1L porcine multiplex antiserum compound preparation

[0028] Heat and dissolve 10g of astragalus polysaccharide in 50ml of water for injection, adjust the pH value between 6 and 7, filter to remove impurities, and then sterilize under high temperature and high pressure at 121°C and 100kPa for 60 minutes, take out the sterilized astragalus polysaccharide and 950ml Porcine multiplex antiserum was mixed, stirred evenly, dispensed into 10ml vials, sealed, and used 60 Co-γ ray irradiation, the irradiation conditions are: irradiation temperature 20°C (or 15°C or 25°C or 17°C), irradiation dose rate 13.8Gy / min (or 5Gy / min or 20Gy / min or 15Gy / min min), the absorbed radiation dose is 12kGy (or 9kGy or 19kGy or 15kGy).

Embodiment 2

[0030] 60 Inactivation of classical swine fever virus in complex preparations of porcine multiple antisera by Co-γ rays

[0031] Take the complex preparation of classical swine fever Shimen blood virus and porcine multi-linked antiserum (the OD value of the classical swine fever virus antibody is 2.860, the OD value of the porcine reproductive and respiratory syndrome virus antibody is 3.042, and the porcine pseudorabies virus antibody OD value is 2.217) 1: 1 mixed, the OD value of the swine fever virus antibody after mixing is 1.896, the OD value of the porcine reproductive and respiratory syndrome virus antibody is 2.214, the OD value of the porcine pseudorabies virus antibody is 1.548, and the minimum lethal dose is 10 5 / ml, packed in aseptic vials, 10ml per bottle, each set of 5 bottles as a group, at the same time set up the poisoned control group, that is, the multi-linked antiserum compound preparation group of non-irradiated pigs. The irradiation temperature is 20°C ...

Embodiment 3

[0033] 60 Inactivation of Foot and Mouth Disease Virus in Porcine Multiple Antiserum Compound Preparation by Co-γ Ray

[0034] Take the composite preparation of foot-and-mouth disease virus OS / 99 strain venom and porcine multi-linked antiserum (the OD value of swine fever virus antibody is 2.860, the OD value of porcine reproductive and respiratory syndrome virus antibody is 3.042, and the OD value of porcine pseudorabies virus antibody is 2.217) 1 : 1 mixed, after mixing, the OD value of swine fever virus antibody was 1.896, the OD value of porcine reproductive and respiratory syndrome virus antibody was 2.214, the OD value of porcine pseudorabies virus antibody was 1.548, and its half infection rate was 10 4 ID 50 / 0.2ml, divided into aseptic vials, 10ml per bottle, each 5 bottles as a group, and the poisoned control group, that is, the multi-linked antiserum compound preparation group of non-irradiated pigs, was set up. The irradiation temperature is 20°C (or 15°C or 25°C...

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Abstract

The invention relates to a method for preparing an antiserum preparation, provides a method for preparing a swine multi-valent antiserum compound preparation, and aims to improve the prevention effect of swine multi-valent antiserum products. The method comprises the following steps of: sterilizing astragalus polysaccharide for 60 minutes at the temperature of 121 DGE C under the pressure of 100kPa; adding the sterilized astragalus polysaccharide solution into antiserum which contains antibodies of swine fever virus, porcine reproductive and respiratory syndrome virus and swine pseudorabies virus to form swine multi-valent antiserum compound solution and making the astragalus polysaccharide account for 1 mass percent of the compound solution; and irradiating the swine multi-valent antiserum compound solution by using 60Co-gamma rays to obtain the swine multi-valent antiserum compound preparation, wherein the irradiation temperature is between 15 and 25 DEG C; the irradiation dose rate is 5 to 20Gy / min; and the irradiation absorbed dose is 9 to 19kGy. The method has the advantages of protecting antibody activity, enhancing autoimmune reaction of organisms, achieving good emergent prevention and immune effect, along with simple and convenient operation and low cost.

Description

technical field [0001] The invention relates to a preparation method of an antiserum preparation, in particular to a preparation method of a porcine multiple antiserum compound preparation. Background technique [0002] Pig multiple antiserum is a comprehensive antibody against certain specific viruses. After injection, it can quickly increase the level of specific virus antibodies in the body, so that the body can obtain passive immunity against these specific viruses in a short period of time. It is the key to these specific viral diseases. emergency immunization measures. However, since there may be exogenous microorganisms including bacteria, viruses and mycoplasma in the pig multiple antiserum, it may bring other bacterial and viral diseases to pigs after use, so an antiserum for viruses, bacteria and mycoplasma, etc. is needed The inactivation technology is used to ensure the safety of porcine multiplex antiserum. [0003] At present, antiserum products mainly use ul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/42A61K47/36A61P31/14A61P31/20A61K31/715
Inventor 乔云龙田晓丽任鸿春苏向花
Owner SHANXI ZHAOYI BIOLOGICAL
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