Preparation method of reagent plate for detecting chloramphenicol in cosmetics
A technology of reagent plate and chloramphenicol is applied in the field of preparation of immunocolloidal gold rapid detection reagent plate, which can solve the problems of high cost, unfavorable sample screening, unoptimistic situation, etc. low cost effect
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[0026] The preparation of reagent plate of the present invention comprises the preparation of chloramphenicol-carrier protein conjugate, the preparation of anti-chloramphenicol monoclonal antibody, the preparation of colloidal gold solution, the preparation of colloidal gold-labeled anti-chloramphenicol monoclonal antibody and chloramphenicol Assembly of the immunocolloidal gold rapid detection reagent plate.
[0027] 1. Coupling of chloramphenicol to carrier protein
[0028] Using carbodiimide (EDC·HCl) method, chloramphenicol was coupled with carrier protein to prepare immune antigen and coating antigen. Weigh 20 mg of bovine serum albumin (BSA), and add 1 mL of distilled water. In addition, 20 mg of EDC·HCl and 20 mg of chloramphenicol were dissolved in 1 mL of distilled water and added to the above solution. After the solution was mixed, it was placed at 4° C. and protected from light for 12 hours (inverted to mix well during this period). Afterwards, they were dialyzed...
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