Biological detoxification technology for rape-seed meal
A rapeseed cake and yeast technology, applied in animal feed, animal feed, application, etc., can solve the problems of large damage to the active ingredients of rapeseed cake, poor palatability of rapeseed cake, and unsatisfactory detoxification effect, and achieve a significant detoxification effect , low cost and low pollution effect
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[0034] 1) Cultivation of strains to be used
[0035] The strains to be used are yeast and lactic acid bacteria;
[0036] Medium formula:
[0037] Wort liquid culture medium: filter malt saccharification liquid (purchased from Xindu Brewery) with 4-6 layers of gauze, if the filtrate is cloudy, use egg white to clarify it, that is, add about 20ml of water to one egg white, and mix thoroughly until foaming So far, then pour it into the saccharification solution, stir and boil, then filter. Dilute the filtrate to 5-6 Baume degrees, pH about 6.4. Sterilize at 121°C for 20 minutes;
[0038] MRS liquid medium: peptone 10g; beef extract 10g; yeast extract 5g; K 2 HPO 4 ·3H 2 O 2g; Sodium acetate 5g; Glucose 20g; Tween 801g; Diammonium citrate 2g; MgSO 4 ·7H 2 O 0.58g; MnSO 4 4H 2 O 0.25g; add distilled water 1000mL; pH6.2-6.4, 121°C, 15min sterilization for later use;
[0039] Training method:
[0040] Saccharomyces: Sterilized and inoculated with wort juice liquid medium, ...
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