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Biological detoxification technology for rape-seed meal

A rapeseed cake and yeast technology, applied in animal feed, animal feed, application, etc., can solve the problems of large damage to the active ingredients of rapeseed cake, poor palatability of rapeseed cake, and unsatisfactory detoxification effect, and achieve a significant detoxification effect , low cost and low pollution effect

Inactive Publication Date: 2010-10-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the more mature methods in the world generally include physical and chemical methods, but the detoxification range of physical and chemical detoxification methods is small, the detoxification rate is low, and the palatability of rapeseed cake after detoxification is poor, so the detoxification effect Not ideal, and these methods have relatively large damage to the active ingredients in the rapeseed cake, or the residue is serious, or the process is more complicated and the cost is higher, all of which have certain shortcomings

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0034] 1) Cultivation of strains to be used

[0035] The strains to be used are yeast and lactic acid bacteria;

[0036] Medium formula:

[0037] Wort liquid culture medium: filter malt saccharification liquid (purchased from Xindu Brewery) with 4-6 layers of gauze, if the filtrate is cloudy, use egg white to clarify it, that is, add about 20ml of water to one egg white, and mix thoroughly until foaming So far, then pour it into the saccharification solution, stir and boil, then filter. Dilute the filtrate to 5-6 Baume degrees, pH about 6.4. Sterilize at 121°C for 20 minutes;

[0038] MRS liquid medium: peptone 10g; beef extract 10g; yeast extract 5g; K 2 HPO 4 ·3H 2 O 2g; Sodium acetate 5g; Glucose 20g; Tween 801g; Diammonium citrate 2g; MgSO 4 ·7H 2 O 0.58g; MnSO 4 4H 2 O 0.25g; add distilled water 1000mL; pH6.2-6.4, 121°C, 15min sterilization for later use;

[0039] Training method:

[0040] Saccharomyces: Sterilized and inoculated with wort juice liquid medium, ...

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Abstract

The invention provides a new method for detoxifying a rape-seed meal by fermenting saccharomycetes and lactic acid bacteria together. The rape-seed meal has been taken as a feed additive for a long time, but the rape-seed meal cannot be used before being detoxified because the rape-seed meal contains constituents, such as glucosinolate and the like, which are harmful to livestock. Microbial fermentation is a novel detoxification method in conventional rape-seed meal detoxification technology and can overcome the defects of small detoxification range, low detoxification rate, poor palatability of detoxified rape-seed meal and the like in physical and chemical detoxification methods. In the invention, glucosinolate in the rape-seed meal is removed by fermenting saccharomycetes and lactic acid bacteria together; after saccharomycetes and lactic acid bacteria are fermented together in a ratio of 1:1 for 3 to 7 days, the detoxification rate can reach about 60 percent, glucosinolate content can be lowered to about 0.15 percent and protein content of the rape-seed meal is remarkably increased due to microbial fermentation.

Description

1. Technical field [0001] The invention utilizes the microbial fermentation method to detoxify the rapeseed cake. 2. Background technology [0002] Rapeseed is one of the main oil crops both in China and in the world. In my country, the output accounts for a quarter of the output of vegetable oil products. China's annual output of rapeseed is more than 8 million tons, ranking first in the world. Rapeseed cake after oil extraction from rapeseed generally accounts for about 60% of the raw material. The huge rapeseed output makes my country have abundant rapeseed cake resources. Rapeseed cake is rich in protein, and its protein content is generally between 30% and 40%, and the highest can reach more than 47%. The amino acid composition of rapeseed protein is reasonable, its lysine content is equivalent to that of soybean, and the content of sulfur-containing amino acids such as lysine and methionine is higher than that of soybean protein. It is the largest high-quality feed p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 杨志荣赵建冯甦张杰侯若彤聂远洋
Owner SICHUAN UNIV
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