Use of young scape as explant for quick propagation and transgenosis of Chinese sacred lily

Abstract

The invention provides use of a young scape as an explant for quick propagation and transgenosis of Chinese sacred lily, namely use of an un-budded young scape in a bulb of Chinese sacred lily as an explant material for in vitro quick propagation and transgenosis. A culture method comprises the following steps of: taking an un-budded flower ball grown by the scape, with the length of 1.5 centimeters; cutting the white part on the lower half part of the young scape into slices of 1 to 1.5 millimeters, and upwards placing the morphological upper end into an adventitious bud induction culture medium; and culturing the slices under light for 12 to 16 hours every day at the culture temperature of between 23 and 25 DEG C. The scape for in vitro quick propagation has the characteristics of high propagation coefficient and the like, and can be used for quick propagation of excellent seed balls. The culture method has the advantages of readily available materials, more material sources and easy establishment of a sterile system with sterilization; and the explant has high-frequency regeneration capacity, the differentiation rate of the adventitious bud reaches over 95 percent after 30 days of culture, and the coefficient of differentiation is over 18, so the scape can replace the currently used bulb disc and be used as the explant for transgenosis and quick propagation and solve the problems of difficult sterilization and low transgenosis efficiency caused by using the bulb disc.

Description

technical field [0001] The invention relates to using young scapes as explants for rapid propagation and transgene of Chinese narcissus, and belongs to the field of biotechnology. Background technique [0002] Chinese narcissus is a traditional famous flower in our country, and it is also an important flower for export and earning foreign exchange. It is famous for its fragrance, beauty, and many flowers. The flowering period of Chinese narcissus coincides with the Spring Festival. As well as its elegant and colorful charm, it is unique among the narcissus flowers and is well-known both at home and abroad. [0003] Chinese narcissus is triploid, so it is difficult to produce new varieties by natural or artificial hybridization. In production, there are problems such as single variety, limited reproduction speed, quality decline caused by virus accumulation, and variety degradation. Transgenic technology will be an effective means of improving Chinese narcissus varie...

AI Technical Summary

Problems solved by technology

[0003] Chinese narcissus is triploid, so it is difficult to produce new varieties by natural or artificial hybridization. In production, there are problems such as single variety, limited reproduction speed, quality decline caused by virus accumulation, and variety degradation.

Transgenic technology will be an effective means of improving Chinese narcissus varieties and cultivating new varieties. However, at present, because bulb discs are mainly used as transgenic explant materials, there are problems such as difficult disinfection, low transformation rate, and difficulty in obtaining transgenic plants, which restrict the use of genetically modified narcissus in China. The Process of Variety Improvement by Engineering Technology

The use of young scapes as explants for in vitro rapid propagation and transgenic Chinese narcissus has not been reported

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