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Antigen antibody complex dissociation liquid kit and application thereof

A technology of antigen-antibody complexes, which is applied in the field of antigen-antibody complex dissociation solution kits and detection kits, can solve the problems that antigen-antibody complex dissociation solution kits have not been reported, and reduce the missed diagnosis rate, The effect of increasing the detection rate and increasing the absolute concentration value

Inactive Publication Date: 2010-09-15
山东莱博生物科技有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, the search results found that the antigen-antibody complex dissociation kit in serum or plasma samples related to the detection has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Preparation of the antigen-antibody complex dissociation solution kit of the present invention

[0015] Prepare 0.3% Triton-X100 (Triton X-100), 1.5% sodium dodecyl sulfate (SDS) and 1.5% 3-[(3-cholanamidopropyl)-dimethyl Ammonium]propanesulfonic acid (3-[(3-cholamidopropyl)-dimethylammonio]propanesulfonic acid) aqueous solution, then mixed together according to the volume of 1:1:1, take the mixed solution and put it into reagent bottles according to 5ml amount, pack Make kits.

Embodiment 2

[0017] Application of the antigen-antibody complex dissociation solution kit of the present invention in the separation of hepatitis C virus antigen-antibody complex

[0018] The kit for detecting HCV core antigen by ELISA can directly detect free core antigen in serum or plasma, which can shorten the "window period" of HCV virus infection detection by about 40-50 days, and detect HCV RNA by nucleic acid detection technology After 1 to 2 days, the presence of HCV core antigen can be detected. However, after the anti-HCV antibody appears in the body, the antibody and the antigen combine to form a complex, and the sensitivity of the enzyme-linked immunosorbent assay for detecting free HCV-cAg is significantly reduced. If the antigen and antibody are combined to form a complex and dissociated, the detection of total HCV-cAg can greatly improve the detection sensitivity.

[0019] The specific dissociation method of the above-mentioned hepatitis C virus antigen-antibody complex is...

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PUM

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Abstract

The invention discloses an antigen antibody complex dissociation liquid kit, which is prepared by blending reagents of 0.3 volume percent of Triton-X100, 1.5 volume percent of lauryl sodium sulfate and 1.5 volume percent of 3-[(3-cholane amidopropyl)-dimethylammonium]propanesulfonic acid in equal amount. The invention also discloses application of the kit in separating a pathogenic antigen antibody complex. The kit of the invention effectively realizes rapid dissociation of the pathogenic antigen antibody complex in a serum or blood plasma sample to be detected, increases the absolute concentration value of an antigen and an antibody, and greatly improves the detection sensitivity of the sample to be detected.

Description

technical field [0001] The invention relates to a detection kit, in particular to an antigen-antibody complex dissociation liquid kit in a serum or plasma sample and its application, and belongs to the technical field of medical detection reagents. Background technique [0002] When the body is infected with a pathogen, the antigen is first produced, and a small amount of antibody is also produced. Antigens can be detected during early infection, and as the infection cycle prolongs, the body’s immune response to pathogens is enhanced, and the amount of antibodies increases accordingly, and combines with antigens to form antigen-antibody complexes, resulting in the accumulation of antigens and antibodies in serum or The concentration in plasma decreases, resulting in a significant decrease in the sensitivity of clinical antigen or antibody detection. Therefore, in order to improve the detection sensitivity of the antigen or antibody in the sample to be tested, it is necessar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/576G01N33/571G01N33/569
Inventor 朱之炜朱翎嘉郑祖惠丁兴龙江长林
Owner 山东莱博生物科技有限公司
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