Method for quantitatively evaluating medicament toxicity by using metabonomic technology

Abstract

The invention discloses a method for quantitatively evaluating medicament toxicity by using metabonomic technology, which is characterized by comprising the following steps: comprehensively and quantitatively measuring small molecule compounds in a biological sample by using the measuring technology based on mass spectrum, nuclear magnetic resonance and the like, then establishing a multi-dimensional spatial mathematical model by adopting a multi-variable data processing method, calculating a relative distance between an administration group and a control group and taking the relative distance as a quantitative index for evaluating the medicament toxicity so as to solve the difficult problem that the medicament toxicity evaluation lacks the quantitative evaluating index. Compared with the conventional toxicity evaluating method, the method has the advantages of wide application, sensitivity, simple and convenient sampling, no harm to the body, and capability of reflecting the toxicity function more comprehensively, and providing a comprehensive and reliable quantitative evaluating method for toxicity evaluation in new medicament research and development and pharmacologic research.

Description

technical field The present invention relates to a new method for quantitative evaluation of drug toxicity, in particular to the quantitative detection of endogenous small molecular compounds in humans, animals or cells by gas chromatography, the calculation of mathematical models by multivariate data processing methods, and the calculation of the comparison between the drug administration group and the control group Distance as a method for quantitative evaluation of drug toxicity. Background technique For a long time, in pharmacological research, the toxicity of many drugs has been evaluated by qualitative rather than quantitative, subjective rather than objective, rough rather than accurate, one-sided rather than comprehensive standards. The specific shortcomings of these methods are mainly reflected in: 1. Lack of quantitative indicators Although some convenient and effective evaluation indicators are used for toxicity evaluation, such as transaminase and white blood c...

AI Technical Summary

Problems solved by technology

The technical problem to be solved by the present invention is: use metabolomics method to comprehensively and quantitatively measure endogenous small molecules in biological samples, and use multivariate data processing method to establish a mathematical model for the obtained data, and calculate the relative ratio between the administration group and the control group. distance, using this quantitative value as an indicator of drug toxicity evaluation, to solve the problem of lack of accurate quantitative evaluation methods for drug toxicity

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