Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Arginine deiminase mutant and preparation and application thereof

A technology for arginine deiminase and mutants, which is applied in the fields of arginine deiminase mutants and their preparation and application, and can solve the problems of uncontrollable modified sites, decreased activity of modified proteins, and unsatisfactory products. Uniform mixture and other problems, to achieve the effect of high activity retention rate, high product uniformity and high in vitro activity

Active Publication Date: 2010-08-25
JIANGSU T MAB BIOPHARMA
View PDF2 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional PEG modification not only leads to a decrease in the activity of the modified protein, but also results in an inhomogeneous mixture of products due to the inability to control the modified site

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Arginine deiminase mutant and preparation and application thereof
  • Arginine deiminase mutant and preparation and application thereof
  • Arginine deiminase mutant and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Embodiment 2

Embodiment 3

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an arginine deiminase mutant with partial mutated lysine and preparation and application thereof. The arginine deiminase mutant of the invention has enzymatic activity of degrading arginine into citruline; compared with the arginine deiminase with the amino acid sequence of SEQ IDNO:1, the amino acid sequence comprises mutants of K9N, K59Q, K66R, K93E, K111R, K119Q, K121Q, K122E, K126E, K178I, K196I and one or more of K209G, K243E, K249D and K279Y. Compared with natural arginine deiminase, after the arginine deiminase mutant of the invention is modified by PEG, the retention rate of active residues is improved; and as the quantity of lysine is reduced, products are more uniform and can be applied to clinical treatment of hepatoma, melanoma and the like.

Description

technical field The invention relates to the field of biotechnology, in particular to an arginine deiminase mutant and its preparation and application. Background technique Arginine is not required for normal cell growth because they can be synthesized from guanidine through a two-step reaction catalyzed by argininosuccinate synthetase (ASS) and argininosuccinate lyase (AL). Arginine: However, hepatocellular carcinomas (HCC), melanomas (melanomas) and some other sarcomas do not express argininosuccinate synthase, so they are auxotrophic for arginine and can only be used in the presence of arginine In the presence of enzymes that degrade arginine, the removal of arginine makes these tumor cells enter a "starvation" state, and their growth is inhibited. Therefore, arginine-degrading enzymes can be used as potential clinical drugs for diseases such as liver cancer and melanoma. Arginine deiminase (arginine deiminase, ADI) can catalyze the conversion of arginine to guanidine, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/78C12N15/55C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A61K38/50A61P31/12A61P35/00A61P25/28
Inventor 黄岩山裘霁宛凌建群温晓芳尚玉栓傅晓瑜范敏王玉姣王叶飞
Owner JIANGSU T MAB BIOPHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com