Experimental method for verifying early process of radiation bystander effect based on arabidopsis thaliana

An experimental method and a technology of shepherd's purse, applied in the fields of biology and radiation detection, can solve the problems of difficult to detect radiation side effect signal transduction and mechanism, etc.

Inactive Publication Date: 2010-08-18
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of radiation side effects early process verification experiment method based on Amaranthia shepherd's purse, take plants as objects, utilize the totipotency of plant cells, in order to solve the research of radiation side effects, it is difficult to detect its radiation side effects at the living body level Signal transduction and mechanism issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Experimental method for verifying early process of radiation bystander effect based on arabidopsis thaliana
  • Experimental method for verifying early process of radiation bystander effect based on arabidopsis thaliana
  • Experimental method for verifying early process of radiation bystander effect based on arabidopsis thaliana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] The present invention comprises the following steps:

[0027] (1) Experimental material cultivation: Arabidopsis seedlings of the AtRAD54Promoter-GUS transgenic line were sterilized and sown on MS medium (3% sugar), cultured vertically under appropriate light intensity and cycle, and the roots of the seedlings were along the surface of the medium. Growing vertically downwards.

[0028] (2) Radiation treatment: Partial irradiation of the root tips of Arabidopsis seedlings by ionizing radiation.

[0029] (3) Cutting and grafting treatment, such as figure 1 Shown:

[0030] A) Cutting treatment: cut off the irradiated part of the root with a scalpel at a specific time point, and transfer the remaining plants to a new MS medium to continue growing.

[0031] B) Grafting treatment: At a specific time point after the irradiation treatment, the irradiated root was cut off with a surgical blade, transferred to a new MS medium, and the root tip was placed vertically downward. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an experimental method for verifying an early process of radiation bystander effect based on arabidopsis thaliana. The method comprises the following steps: adopting model plant arabidopsis thaliana as a material; carrying out ionizing radiation to the arabidopsis thaliana; then carrying out cutting and grafting treatment; extracting the AtRAD54 gene of new arabidopsis thaliana seedling which is grafted to the root tip of the radiated arabidopsis thaliana; and taking the expression level of the AtRAD54 gene as a detection end point. The innovation of the method lies in overcoming the limitation that the separation and connection of a radiated tissue (organ) and a non-radiated tissue (organ) can not be realized in animal experiments by adopting the characteristics of being capable of being cut and grafted which are caused by totipotency of a plant cell and providing a novel method system for studying the early process of remote radiation signals at the individual level.

Description

technical field [0001] The invention relates to the fields of biology and radiation detection, in particular to an experimental method for verifying the early process of radiation bystander effects based on Amaranthus shepherd's purse. Background technique [0002] In the traditional sense, the principle of radiation effect is that the energy of ionizing radiation is deposited in the radiation target (such as DNA, protein, etc.), which induces radiation damage, and then produces subsequent biological effects. However, studies in recent years have found that radiation effects are not limited to cells directly irradiated, and unirradiated bystander cells also exhibit biological effects similar to those of irradiated cells, that is, radiation induced bystander effect. , RIBE). [0003] In the 16 years since the discovery of radiation bystander effects in 1992, the research system of radiation bystander effects has mainly been in vitro cells. The research system is mainly divi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 王婷李方华卞坡吴跃进
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap