Method for efficiently propagating torch pineapples by utilizing in-vitro leaves

Abstract

The invention provides a method for efficiently propagating torch pineapples by utilizing in-vitro leaves, which comprises the following steps: using inner layer young leaves of strong side lateral buds of the torch pineapples as explants; carrying out explant selection, leaf disinfection, leaf inducement culture, adventitious bud multiplication successive transfer culture and adventitious bud rooting culture for forming complete plant seedlings; and domesticating and transplanting the complete plant seedlings into a seedling culture substrate for growing the plant seedlings into normal torch pineapple plants. The method for efficiently propagating the torch pineapples by utilizing the in-vitro leaves of the invention has the bud differentiation rate of 74.7 percent, each leaf has the differentiation bud number of 5.2, the multiplication coefficient is 3.6, in addition, the buds grow strongly, the rooting rate reaches 85.5 percent, and the transplanting survival rate reaches 95 percent. The method for efficiently propagating the torch pineapples by utilizing the in-vitro leaves of the invention realizes the storage on the single strains of the torch pineapples, overcomes the defects of overlong seedling culture period, low propagation coefficient and poor seedling consistency of a traditional propagating method, and can realize the factory large-scale production of the seedling for adapting to the requirements of the market on the torch pineapples.

Description

technical field [0001] The invention relates to a method for efficiently propagating torch pineapple by using isolated leaves, specifically, a method for rapidly obtaining torch pineapple plants by using tender inner slices of strong lateral buds of the torch pineapple as explants and culturing them on a culture medium. Background technique [0002] The torch pineapple (Guoziman conifera) is a fruit vine of the Bromeliaceae family, native to tropical and subtropical regions of Central and South America. Torch pineapple is a perennial evergreen foliage plant with a plant height of 30-50cm. The leaves are wide and linear, bright in color, thick in texture, and have no thorns on the edge of the leaves. The leaves are clustered on the shortened stems to form leaf cups for water storage and absorption. nutrient. The upright flower stems are pulled out from the center of the leaf clusters. The style stands upright in the radiant and green sword leaf cluster, unbranched. The inflo...

AI Technical Summary

Problems solved by technology

However, if the stem tip of the mother plant is used for propagation, the number of explants is limited, and it is easy to damage the individual mother plant. When the shoot tip of the lateral bud is used for propagation, the success rate of disinfection of the lateral buds is extremely low, and the bud initiation period is as long as 90-150 days. The cycle is too long, the reproduction coefficient is low, the market price is high, and it is difficult to meet the market demand. Therefore, it is urgent to study a method that can quickly produce a large number of torch pineapple.

[0003] Tissue culture is an important way to rapidly propagate fine plant varieties. There have been some reports on tissue culture of certain species of plants of the genus Prunus in Bromeliaceae, but most of them use seeds or shoot tips for propagation, and use young lateral buds to reproduce. Tissue culture of young leaves but no related reports