Method for rapidly growing and cultivating dendrobium officinale
A cultivation method, the technology of Dendrobium candidum, applied in cultivation, soilless cultivation, botanical equipment and methods, etc., can solve the problems of soaring price, low survival rate of seedling transplanting, and low active ingredients, so as to reduce costs, The effect of shortening the tissue culture time
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Embodiment 1
[0044] When flowering, a strong mother plant is selected for artificial pollination, and the fruit is ripe 120 days after pollination, and the fruit is used as an explant for sowing. When sowing, the explants were soaked in alcohol with a volume fraction of 7% for 30 seconds, sterilized in a mercuric solution with a mass fraction of 0.1% for 20 minutes, rinsed with sterile water for 4 times, then cut the fruit, and used an inoculation needle to inoculate the yellow-white Powdered embryos are inoculated into the seed germination medium (each liter of the medium contains Huabao No. 1 1.5g protein, 0.5g coconut milk, 50mL, sucrose 15g, agar 6g, activated carbon, and the rest are vitamins and inositol in MS medium. Components), the seeds germinated after 10 days to form protocorms, and formed seedlings after 80 days.
[0045] Transfer 100 plants of 2-3cm high seedlings to the test tube flowering medium (every liter contains 0.5 mg of 6-carbyl acetic acid (6-BA), 0.1 mg of naphthal...
Embodiment 2
[0048] When flowering, select a strong mother plant for artificial pollination, and the fruit is ripe 110 days after pollination, and the fruit is used as an explant for sowing; Apply 0.15% mercuric chloride solution for disinfection for 30 minutes, rinse with sterile water for 5 times, then cut the fruit, and use an inoculation needle to inoculate the yellow-white powdery embryos into the seed germination medium (each liter of culture medium contains Huabao 1- Snake, protein refining 1g, coconut milk 80mL, sucrose 25g, agar 6.5g, gac 0.8g, all the other are the vitamin and inositol composition in the MS medium), after 10 days, the seeds germinate and form protocorms. Obtain more seedlings, form protocorms into buds and protocorm-like mixed tissues, in the subculture propagation medium (every liter contains 1g of Huabaokong, 0.5g of protein refining, 50mL of coconut milk, 10g of sucrose, 6g of agar, Activated charcoal 1g, 6-taro base howling 0.5mg, all the other are vitamins a...
Embodiment 3
[0051] When flowering, a strong mother plant is selected for artificial pollination, and the fruit is ripe 130 days after pollination, and the fruit is used as an explant for sowing. When sowing, the explants were soaked in alcohol with a volume fraction of 80 for 60 seconds, sterilized in a 0.2% mercuric solution for 30 minutes, rinsed with sterile water for 4 times, and then the fruit was cut open, and the yellow-white powder was inoculated with an inoculation needle. Embryos are inoculated into the seed germination medium (the medium contains 1.5g of Huabao No. 1 per liter, 100mL of protein refining 2g coconut milk, 30g of sucrose, 7g of agar, activated carbon, and the rest are vitamins and inositol components in the Ms medium. ), after 10 days, the seeds germinate and form protocorms. In order to apply for more seedlings in the production process, the protocorms are formed into a mixed tissue of buds and protocorms, and the protocorms are used in the subculture proliferatio...
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