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Method for rapidly growing and cultivating dendrobium officinale

A cultivation method, the technology of Dendrobium candidum, applied in cultivation, soilless cultivation, botanical equipment and methods, etc., can solve the problems of soaring price, low survival rate of seedling transplanting, and low active ingredients, so as to reduce costs, The effect of shortening the tissue culture time

Inactive Publication Date: 2010-08-18
WUHAN ZHONGDA RUIFENG BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Dendrobium officinale is commonly known as immortal grass in the world. Its main components are alkaloids and polysaccharides. It is also rich in amino acids and trace elements. Modern clinical application and medicinal research have confirmed that Dendrobium officinale can treat throat diseases, gastrointestinal diseases, cataracts, and cardiovascular diseases. , diabetes and inhibition of tumor growth have significant curative effects. The demand in the domestic and foreign markets continues to increase, and the price has soared. Due to the slow growth and low output of wild Dendrobium candidum, coupled with people's continuous digging, wild Dendrobium candidum is facing extinction. It cannot meet the market demand. The Chinese pharmaceutical industry lists Dendrobium candidum as a kind of key protected rare and endangered species, and encourages artificial large-scale cultivation of Dendrobium candidum. At present, some scientific research institutions and business units in China are focusing on tissue culture and artificial large-scale cultivation of Dendrobium candidum Cultivation techniques have been explored and tested, and some achievements have been made. However, raw materials such as chemicals, chemicals, and pesticides were used during the tissue culture and cultivation period, and there are still problems such as slow propagation speed of Dendrobium candidum seedlings, and the survival rate of tissue cultured seedlings and trained seedlings transplanted. The main problems such as low growth rate, low yield, residual chemical components, and low effective components have seriously restricted the industrialization and large-scale development of Dendrobium officinale.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] When flowering, a strong mother plant is selected for artificial pollination, and the fruit is ripe 120 days after pollination, and the fruit is used as an explant for sowing. When sowing, the explants were soaked in alcohol with a volume fraction of 7% for 30 seconds, sterilized in a mercuric solution with a mass fraction of 0.1% for 20 minutes, rinsed with sterile water for 4 times, then cut the fruit, and used an inoculation needle to inoculate the yellow-white Powdered embryos are inoculated into the seed germination medium (each liter of the medium contains Huabao No. 1 1.5g protein, 0.5g coconut milk, 50mL, sucrose 15g, agar 6g, activated carbon, and the rest are vitamins and inositol in MS medium. Components), the seeds germinated after 10 days to form protocorms, and formed seedlings after 80 days.

[0045] Transfer 100 plants of 2-3cm high seedlings to the test tube flowering medium (every liter contains 0.5 mg of 6-carbyl acetic acid (6-BA), 0.1 mg of naphthal...

Embodiment 2

[0048] When flowering, select a strong mother plant for artificial pollination, and the fruit is ripe 110 days after pollination, and the fruit is used as an explant for sowing; Apply 0.15% mercuric chloride solution for disinfection for 30 minutes, rinse with sterile water for 5 times, then cut the fruit, and use an inoculation needle to inoculate the yellow-white powdery embryos into the seed germination medium (each liter of culture medium contains Huabao 1- Snake, protein refining 1g, coconut milk 80mL, sucrose 25g, agar 6.5g, gac 0.8g, all the other are the vitamin and inositol composition in the MS medium), after 10 days, the seeds germinate and form protocorms. Obtain more seedlings, form protocorms into buds and protocorm-like mixed tissues, in the subculture propagation medium (every liter contains 1g of Huabaokong, 0.5g of protein refining, 50mL of coconut milk, 10g of sucrose, 6g of agar, Activated charcoal 1g, 6-taro base howling 0.5mg, all the other are vitamins a...

Embodiment 3

[0051] When flowering, a strong mother plant is selected for artificial pollination, and the fruit is ripe 130 days after pollination, and the fruit is used as an explant for sowing. When sowing, the explants were soaked in alcohol with a volume fraction of 80 for 60 seconds, sterilized in a 0.2% mercuric solution for 30 minutes, rinsed with sterile water for 4 times, and then the fruit was cut open, and the yellow-white powder was inoculated with an inoculation needle. Embryos are inoculated into the seed germination medium (the medium contains 1.5g of Huabao No. 1 per liter, 100mL of protein refining 2g coconut milk, 30g of sucrose, 7g of agar, activated carbon, and the rest are vitamins and inositol components in the Ms medium. ), after 10 days, the seeds germinate and form protocorms. In order to apply for more seedlings in the production process, the protocorms are formed into a mixed tissue of buds and protocorms, and the protocorms are used in the subculture proliferatio...

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PUM

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Abstract

The invention discloses a method for rapidly growing and cultivating dendrobium officinale, which comprises the following steps of: taking back mature shadow fruit seeds in the country of origin for wild dendrobium officinale, and placing the seeds in a clean culture dish; after natural crack, pouring the seeds into a clean beaker, sealing the clean beaker, and storing the beaker in a low temperature box; performing a certain sterilization procedure on the stored seeds, placing the seeds in an MS minimal medium, performing seed germination cultivation, wherein the seeds gradually become green and form protocorm, and observing that needles appear in the protocorm by using magnifying glass; transferring the protocorm into the MS minimal medium, performing protocorm proliferation and differentiation culture, observing that plantlets are differentiated from the protocorm in a bottle along the production of partial new protocorm; and transferring the plantlets into the MS minimal medium, and performing strong seedling and rooting culture, seedling hardening and transplanting. The survival rate is over 97 percent without the seedling hardening process, and the yield is doubled. The growth speed is improved by 50 percent, the content of each component is equivalent to that of the wild dendrobium officinale, and the contents of amino acid and microelement are higher than those of the wild dendrobium officinale.

Description

technical field [0001] The invention belongs to the field of propagation of dendrobium candidum, and more specifically relates to a method for rapid growth and cultivation of dendrobium candidum. Background technique [0002] Dendrobium officinale is commonly known as human fairy grass. Its main components are alkaloids and polysaccharides. It is also rich in amino acids and trace elements. Modern clinical applications and medicinal studies have confirmed that Dendrobium officinale can treat throat diseases, gastrointestinal diseases, cataracts, and cardiovascular diseases. , diabetes and inhibition of tumor growth have significant curative effects. The demand in the domestic and foreign markets continues to increase, and the price has soared. Due to the slow growth and low output of wild Dendrobium candidum, coupled with people's continuous digging, wild Dendrobium candidum is facing a state of extinction. It cannot meet the market demand. The Chinese pharmaceutical industr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G9/00A01G31/00C12N5/04
Inventor 朱光艮
Owner WUHAN ZHONGDA RUIFENG BIOENG
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