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Cryopreservation method for oocyte / embryo and frozen carrier thereof

A technology of vitrification and preservation methods, which is applied to the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of difficult operation and not suitable for beginners, so as to facilitate storage and avoid high costs and tediousness. program, efficient effect

Inactive Publication Date: 2010-07-21
CHINA AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The advantage of this method is that the semi-thin tube makes the freezing liquid directly contact with liquid nitrogen, which greatly improves the freezing speed, and has the advantage of being easy to store; the disadvantage is that the operation is difficult, and skilled technicians are required, so it is not suitable for beginners

Method used

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  • Cryopreservation method for oocyte / embryo and frozen carrier thereof
  • Cryopreservation method for oocyte / embryo and frozen carrier thereof

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Embodiment Construction

[0021] The present invention will be further elaborated below by specific examples.

[0022] (1) EFS30-40 vitrification storage solution, freezing pretreatment solution and thawing solution.

[0023] ① EFS30~40 vitrification storage solution: take PBS as the base solution, add polysucrose, sucrose and ethylene glycol, and prepare polysucrose with a volume ratio of 30%, sucrose with a concentration of 0.5mol / L, and ethylene glycol with a volume ratio of 30%. It is 30%-40% EFS30-40 cryopreservation solution.

[0024] ② The freezing pretreatment solution is 5% to 10% ethylene glycol (Ethylene glycol: EG): PBS is used as the base solution, and ethylene glycol is added to prepare a 5% to 10% ethylene glycol freezing pretreatment solution by volume. treatment fluid.

[0025] ③ The thawing solution is 0.3-0.5 mol / L sucrose solution: use PBS as the base solution to prepare 0.3-0.5 mol / L sucrose solution as the thawing solution.

[0026] (2) Freezing: At room temperature (20-25°C), ...

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Abstract

The invention relates to a method for conserving human bodies, animals or parts thereof and a frozen carrier thereof, in particular to a cryopreservation method for oocyte / embryo and a frozen carrier thereof. The method comprises the following steps: a step for preparing refrigerating fluid, a step for preparing thawing solution, a step for freezing and a step for thawing. The method is characterized in that the frozen carrier used in the step for freezing and the step for thawing is a frozen net. The frozen net includes a net body and a net handle. The invention provides the cryopreservation method for the oocyte or the embryo and the frozen carrier thereof. The method is characterized by simple operation, fast speed and high efficiency.

Description

technical field [0001] The invention relates to a preservation method for human body, animal or its part and its freezing carrier, in particular to a vitrification freezing preservation method of oocyte / embryo and its freezing carrier. Background technique [0002] Since Rall et al. (1985) invented the embryo vitrification cryopreservation method, the cryopreservation can be performed at room temperature without the aid of a freezer, and the procedure has been greatly simplified. After improvement by many researchers, the current oocyte / embryo vitrification method only takes about 1 minute to complete a procedure. During this process, many researchers creatively invented many frozen carrying objects, which were named in various ways. The following methods are commonly used today: Nylon Ring Vitrification (Cryoloop), Open Pulled Straw (OPS, Open Pulled Straw), Fine Glass Tube (GMP), Microdrops, Semi-thin Tube Law (hemi-straw). [0003] Nylon ring vitrification method (Cryo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 朱士恩李俊杰王彦平侯云鹏
Owner CHINA AGRI UNIV
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