Compound biological soil improver and preparation method thereof
A technology of biological soil improvement and soil improver, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of agricultural product quality decline, groundwater pollution, environmental pollution, etc., and increase crop yield and infection rate. Reduced, significant effect
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Embodiment 1
[0017] Prepare LB medium in two 200L fermenters, sterilize at 121°C-125°C for 15-20 minutes, inoculate 10% of Paenibacillus polymyxa and Bacillus subtilis respectively, pass in sterilized air, and culture at 40°C After 20 hours, the number of viable bacteria per milliliter is greater than 1×10 11 Each, each 1Kg of bacteria sludge is centrifugally extracted at a speed of 12000 rev / min on a centrifuge.
[0018] Mix 100g of yeast powder, 100g of seaweed polysaccharide powder, 200g of skimmed milk powder and 20g of sodium chloride to form a protective agent, add appropriate amount of sterile water, add Paenibacillus polymyxa and Bacillus subtilis sludge successively, stir and emulsify at low speed Then pack into a freeze-drying tray, freeze-dry in a vacuum dryer, and obtain Paenibacillus polymyxa and Bacillus subtilis freeze-dried powder 2.2Kg altogether.
[0019] Take 1.0Kg of the above compound biological bacteria as the core, add 300Kg of fermented organic fertilizer from live...
Embodiment 2
[0021] In two 500L fermentors, configure LB medium respectively, sterilize at 121°C-125°C for 15-20 minutes, inoculate 10% Paenibacillus polymyxa and potassium bacteria-jelly-like Bacillus (Bacillus mucilaginosus) respectively, Introduce sterile air, and after 20 hours of incubation at 40°C, the number of viable bacteria per milliliter is greater than 1×10 11 One, each 2Kg of bacteria sludge is centrifugally extracted with the rotating speed of 12000 revs / min on the centrifuge.
[0022] Mix 400g of yeast powder, 300g of seaweed polysaccharide powder, 500g of skimmed milk powder and 30g of sodium chloride to form a protective agent with an appropriate amount of sterile water, and then add Paenibacillus polymyxa sludge and potassium bacteria-jelly-like Bacillus sludge After low-speed stirring and emulsification, put it into a freeze-drying tray, and freeze-dry it in a vacuum dryer to obtain a total of 3.0 Kg of Paenibacillus polymyxa and potassium bacteria-Bacillus jellylike fre...
Embodiment 3
[0025] Obtain Paenibacillus polymyxa and Bacillus subtilis two bacterium freeze-dried powder composite biological bacteria 1.2Kg by the method for embodiment 1.
[0026] Take 0.3Kg of the above-mentioned composite biological bacteria as the core, add 150Kg of livestock and poultry manure fermented organic fertilizer and 150Kg of perlite to mix, and obtain 295Kg of composite biological soil improver after screening, wherein the organic matter content is 21%, and the number of biological viable bacteria is 2.6×10 9 pcs / g.
[0027] Beneficial effects of the present invention are further elaborated below by test example:
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