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Method for preparing rice protein with high dissolubility

A rice protein, soluble technology, applied in the field of deep processing of plant protein resources, to achieve the effect of improving comprehensive utilization rate and added value, and broadening the application field

Inactive Publication Date: 2010-06-02
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And at present, there is no large-scale production of rice protein products in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Separation of rice protein by alkali extraction and acid precipitation

[0035] Broken rice → Refining [solid-liquid ratio 1:6 (w / v)] → Adjust pH to 12.0 with NaOH → Extract at room temperature for 2 hours →

[0036] Centrifuge at 3000r / min for 15min→take the supernatant→adjust the pH to 4.8 with HCl→stand still for 30min→centrifuge at 4000r / min for 10min→collect the precipitate→wash (solid-liquid ratio 1:10)→centrifuge at 4000r / min for 10min→collect the precipitate→separate rice protein

[0037] (2) Isolation of rice protein complex enzyme multi-site directed modification

[0038] Separation of rice protein → pulping according to protein: water = 1:9 (w / v) → rice protein suspension → adjusting pH to 8.5 with NaOH → adding 1.5% (w / w) compound enzyme (alkaline protease: trypsin: papaya Protease=1.00:0.75:0.50)→50°C enzymatic hydrolysis for 4h→85°C / 10min to inactivate enzyme→3000r / min centrifugation for 15min→collect supernatant→enzymatically modified rice protein l...

Embodiment 2

[0044] (1) Separation of rice protein by alkaline extraction and acid precipitation

[0045]Broken rice → Refining [solid-liquid ratio 1:5 (w / v)] → Adjust pH to 12.0 with NaOH → Extract at room temperature for 2 hours →

[0046] Centrifuge at 3000r / min for 15min→take the supernatant→adjust the pH to 4.8 with HCl→stand still for 30min→centrifuge at 4000r / min for 10min→collect the precipitate→wash (solid-liquid ratio 1:10)→centrifuge at 4000r / min for 10min→collect the precipitate→separate rice protein

[0047] (2) Isolation of rice protein complex enzyme multi-site directed modification

[0048] Separation of rice protein → pulping according to protein: water = 1:10 (w / v) → rice protein suspension → adjusting pH to 8.5 with NaOH → adding 2.0% (w / w) compound enzyme (alkaline protease: trypsin: papaya Protease=1.10:0.70:0.45)→50°C enzymatic hydrolysis for 4.5h→85°C / 10min to inactivate enzyme→3000r / min centrifugation for 15min→collect supernatant→enzymatically modified rice prote...

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Abstract

The invention relates to a method for preparing rice protein with high dissolubility, and belongs to the technical field of deep machining of plant protein resource. The method uses broken rice as a raw material; and the rice protein separated out by an alkaline extraction and acid precipitation method is subject to working procedures of compound enzyme multi-locus directed modification, graft copolymerization with amylose, spraying, drying and the like to prepare the rice protein with high dissolubility. The nitrogen solubility index NSI of the rice protein prepared by the method is more than or equal to 85 percent; and the rice protein can be widely used as a functional ingredient of a protein beverage, a roasted product, a minced meat product and a nutritional health-care product.

Description

technical field [0001] The invention discloses a method for preparing highly soluble rice protein, which belongs to the technical field of deep processing of plant protein resources. Background technique [0002] Rice protein is a high-quality vegetable protein resource. Its essential amino acid content is close to the recommended model of FAO / WHO. Its biological value ranks first among cereal grains, and its digestibility is as high as 85%. At the same time, as a hypoallergenic protein resource, it is especially suitable as the base material of nutritional food for infants and special populations. However, there is no large-scale production of rice protein products in China at present. This is because the rice protein prepared by the traditional extraction method has always had problems such as low extraction rate, low purity, and poor solubility in the range of pH 4-10. The rice protein reported here is The nitrogen solubility index (NSI) of NSI is generally less than 50%...

Claims

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Application Information

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IPC IPC(8): C12P21/06C08G81/00C08B37/00C08H1/00
Inventor 程云辉郭建伟银波盛灿梅
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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