Chemiluminescence method for detecting organophosphorus pesticide

Abstract

The invention discloses a chemiluminescence method for detecting an organophosphorus pesticide, and belongs to the field of pesticide residue analysis and the technical field of chemiluminescence analysis. The method comprises the following steps: 1, selecting a chemiluminescence system; 2, selecting a sensitizer; 3, performing single factor and orthogonal tests; 4, performing interference tests; 5, drawing a standard curve; 6, analyzing a sample; 7, researching a chemiluminescence mechanism; and 8, researching a relationship between a structure of the pesticide and chemiluminescence. The method has the advantages of simple and quick operation, wide linear range and low cost; an inorganic salt NaCl serves as the sensitizer of the system for the first time so as to improve the sensitivity of the system; and the method is suitable for detecting residue of organophosphorus pesticides with similar structures.

Description

technical field [0001] The invention belongs to the field of pesticide residue analysis and the technical field of chemiluminescence analysis, in particular to a chemiluminescence method for detecting organophosphorus pesticides. Background technique [0002] Organophosphorus pesticides are organic compounds containing C-P bonds, or C-O-P, or C-S-P, or C-N-P bonds. In 1938, Germany discovered that organic phosphorus had a strong insecticidal effect, and it began to be widely used in agriculture. my country began to develop and produce organophosphorus pesticides in 1956, and has now been able to produce more than 40 varieties to supply the domestic market, and has become one of the world's largest pesticide producers. Since my country banned organochlorine pesticides such as HCH and DDT in 1983, organophosphorus pesticides have become the most widely used pesticides in my country. At present, the types of pesticides in my country's pesticide market mainly include organopho...

AI Technical Summary

Problems solved by technology

However, the production of immunogen is complicated and difficult to obtain, so the immunoassay method has certain limitations in the application of the determination of organophosphorus pesticides

[0009] 4. Although the use of biosensors to measure organophosphorus pesticides is simple, fast, and sensitive, acetylcholinesterase has the same inhibitory effect on the same type of organophosphorus pesticides, so the selectivity of this type of sensor is not high. And the enzyme is very unstable. Inactivation phenomenon, the inhibition of enzymes by organophosphorus pesticides is irreversible, the enzyme electrodes cannot be reused, and the cost is extremely high. The enzyme inhibition method has low sensitivity and is generally only suitable for qualitative and semi-quantitative detection.

But this patent has the following defects: what the patent declares can be used for the determination of the total amount of organophosphorus pesticide residues on vegetables or fruits, but there are many types of organophosphorus pesticides, and this patent only provides chemical compounds for trichlorfon, dichlorvos, and omethoate Luminescence detection does not determine whether other organophosphorus pesticides with different chemical structures can be detected, so it cannot be generally considered that the total amount of residues can be detected

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